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thioflavine T stain <technique> A stain employed to detect amyloid, which induces specific yellow fluorescence; tissue sections are first put in alum-haematoxylin to quench nuclear fluorescence and then stained in thioflavine T.
(05 Mar 2000)
Ehrlich's acid haematoxylin stain <technique> An alum type of haematoxylin stain used as a regressive staining method for nuclei, followed by differentiation to required staining intensity; the solution may be allowed to ripen naturally in sunlight or partially oxidised with sodium iodate.
(05 Mar 2000)
Ehrlich's aniline crystal violet stain <technique> A stain for Gram-positive bacteria.
(05 Mar 2000)
Ehrlich's triacid stain <technique> A differential leukocytic stain comprised of saturated solutions of orange G, acid fuchsin, and methyl green.
(05 Mar 2000)
Ehrlich's triple stain <technique> A mixture of indulin, eosin Y, and aurantia.
(05 Mar 2000)
Einarson's gallocyanin-chrome alum stain <technique> A method for staining both RNA and DNA a deep blue; with proper controls, nucleic acid content of stained cells and nuclei may be estimated by cytophotometry; also useful for Nissl substance.
(05 Mar 2000)
Tizzoni's stain <technique> A stain used as a test for iron in tissue; the tissue is treated with a solution of potassium ferrocyanide and then with dilute hydrochloric acid; a blue colouration indicates the presence of iron.
(05 Mar 2000)
Toison's stain <technique> A blood diluent and leukocyte stain containing methyl violet, sodium chloride, sodium sulfate, and glycerin; also used for erythrocyte counts.
(05 Mar 2000)
trichrome stain <technique> Staining combinations which usually contain three dyes of contrasting colours selected to stain connective tissue, muscle, cytoplasm, and nuclei in bright colours; generally, tissue sections are first dyed in iron haematoxylin before being treated with the other dyes.
(05 Mar 2000)
Eranko's fluorescence stain <technique> Exposure of frozen sections to formaldehyde which produces a strong yellow-green fluorescence from cells containing norepinephrine.
(05 Mar 2000)
trypsin G-banding stain <technique> A unique chromosome staining technique, used in human cytogenetics to identify individual chromosomes, which produces characteristic bands.
It utilises acetic acid fixation, air drying, denaturing chromosomes mildly with proteolytic enzymes, salts, heat, detergents, or urea, and finally Giemsa stain; chromosome bands appear similar to those fluorochromed by Q-banding stain.
Synonym: Giemsa chromosome banding stain.
(05 Mar 2000)
Jenner's stain <technique> A methylene blue eosinate similar to Wright's stain but differing in not using polychromed methylene blue; used for staining of blood smears.
(05 Mar 2000)
Kasten's fluorescent Feulgen stain <technique> A fluorescent modification of the Feulgen stain, utilizing any one of a variety of fluorescent basic dyes to which SO2 is added; the brilliant fluorescence makes this method unusually sensitive and adaptable to cytofluorometric quantification of DNA.
(05 Mar 2000)
Kasten's fluorescent PAS stain <technique> A fluorescent modification of the periodic acid Schiff stain for polysaccharides which uses one of Kasten's fluorescent Schiff reagents.
(05 Mar 2000)
Ziehl-Neelsen stain <technique> A method for staining acid-fast bacteria using Ziehl's stain, decolorizing in acid alcohol, and counterstaining with methylene blue; acid-fast organisms appear red, other tissue elements light blue; a modification of this stain is also used for Actinomycetes and Brucella.
(05 Mar 2000)
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