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Dane's stain <technique> A stain for prekeratin, keratin, and mucin which employs haemalum, phloxine, Alcian blue, and orange G; nuclei appear orange to brown, acid mucopolysaccharides pale blue, and keratins orange to red-orange.
(05 Mar 2000)
DAPI stain <technique> A sensitive fluorescent probe for DNA, 4'6-diamidino-2-phenylindole-2HCl, used in fluorescence microscopy to detect DNA in yeast mitochondria, chloroplasts, viruses, mycoplasma, and chromosomes; DNA is visualised in vitally stained living cells and after cells are fixed in formaldehyde.
(05 Mar 2000)
supravital stain <technique> A procedure in which living tissue is removed from the body and cells are placed in a nontoxic dye solution so that their vital processes may be studied.
(05 Mar 2000)
diazo stain <technique> For argentaffin granules, in enterochromaffin cells, a variety of diazonium salts are used to blacken the cells.
(05 Mar 2000)
Dieterle's stain <technique> Stain used to demonstrate spirochetes and Leishman-Donovan bodies; employs silver nitrate and uranium nitrate.
(05 Mar 2000)
differential stain <technique> A dye used to colour one portion of a tissue or cell which remained unaffected when the other part was stained by a dye of different colour.
Synonym: differential stain.
(05 Mar 2000)
immunofluorescent stain <technique> Stain resulting from combination of fluorescent antibody with antigen specific for the antibody portion of the fluorochrome conjugate.
(05 Mar 2000)
India ink capsule stain <technique> A negative stain for crystal bacteria in which cells appear purple (Gram's crystal violet) and the capsules appear clear against a dark background.
(05 Mar 2000)
quinacrine chromosome banding stain q-banding stain
intravital stain <technique> A stain which is taken up by living cells after parenteral administration, e.g., intravenously or subcutaneously.
(05 Mar 2000)
iodine stain <technique> A stain to detect amyloid, cellulose, chitin, starch, carotenes, and glycogen, and to stain amoebas by virtue of their glycogen; faeces and other wet preparations are stained directly with Lugol's iodine solution; smears are treated with Schaudinn's fixative and then stained with alcoholic iodine, followed by Heidenhain's iron haematoxylin.
(05 Mar 2000)
Orth's stain <technique> A lithium carmine stain for nerve cells and their processes.
(05 Mar 2000)
Taenzer's stain <technique> An orcein solution used for staining elastic tissue.
Synonym: Unna-Taenzer stain.
(05 Mar 2000)
Takayama's stain <technique> A stain containing pyridine, sodium hydrate, and dextrose; used for identification of blood stains; a drop added to a suspected blood stain results in the formation of haemochromogen crystals.
(05 Mar 2000)
telomeric R-banding stain <technique> A modified R-banding stain in which the telomeres become strongly stained and faint R-banding still occurs over the rest of the chromosomes; uses air-dried slides, aging for several days, and staining in hot phosphate-buffered Giemsa stain.
(05 Mar 2000)
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