| technology, recombinant DNA | A series of procedures used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed (recombined) from segments from 2 or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, autonomously (on its own) or after it has become integrated into a chromosome. (12 Dec 1998) |
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| Tet DNA polymerase | <enzyme> From thermus thermophilus; thermostable, used as a replacement for taq polymerase in DNA sequencing Registry number: EC 2.7.7.- Synonym: DNA polymerase tet (26 Jun 1999) |
| transfer DNA | <molecular biology> The transforming region of DNA in the Ti plasmid of agrobacterium tumefaciens. Acronym: t-DNA (04 Jul 1999) |
| triple DNA | <molecular biology> A variation of DNA, in which there is a third strand of nucleotides binding to the first two by specific base pairing. (14 Nov 1997) |
| exogenous DNA | <molecular biology> DNA originating outside an organism. (09 Oct 1997) |
| junk DNA | That portion of DNA which is not transcribed and expressed, comprising about 90% of the 3 billion base pairs of the human genome; its function is not known. (05 Mar 2000) |
| extrachromosomal DNA | DNA that occurs naturally outside of the nucleus (e.g., mitochondrial DNA). (05 Mar 2000) |
| UlTma DNA polymerase | <enzyme> A thermostable DNA polymerase with putative proofreading activity Registry number: EC 2.7.7.- (26 Jun 1999) |
| Z-DNA | <molecular biology> A form of DNA, or a region of DNA, in which the helix is flipped into a lefthanded helix, characterised by alternating purines and pyrimidines. This region is thought to be the target of a DNA-binding protein. (20 Jun 2000) |
| zero time-binding DNA | DNA that has become the duplex form at the start of a reassociation process. Acronym: DNA (05 Mar 2000) |
| unique DNA | <molecular biology> Any nucleotide sequence in DNA that is found only once in a given genome. (09 Oct 1997) |
| unscheduled DNA synthesis | <cell biology, molecular biology> Any synthesis of DNA that happens ouside the S phase of the cell cycle. (13 Nov 1997) |
| uracil-DNA glycosidase | <enzyme> Catalyses hydrolysis of n-glycosidic bond of damaged nucleotide residues to give free uracil and partly depyrimidinated DNA of unaltered chain length; does not cleave phosphodiester bonds; a DNA repair enzyme Registry number: EC 3.2.2.- Synonym: ura-DNA glycosidase, uracil-DNA glycosylase, uracil n-glycosidase, ura-DNA glycosylase, uracil n-glycosylase (26 Jun 1999) |
| foldback DNA | <molecular biology> Sections of single-stranded DNA which have sequences that are palindromes, or which read the same both backwards and forwards, that may base pair with each other instead of with the right sections of the other strand during renaturation into double-stranded DNA. (09 Oct 1997) |
| 5-methylcytosine-DNA glycosylase | <enzyme> Binds equally well to methylated and non-methylated DNA; isolated from developing chicken embryos and mouse fibroblasts; mw 52.5 kD Registry number: EC 3.2.2.- (26 Jun 1999) |