| RT-PCR | Reverse transcription followed by the polymerase chain reaction |
|---|---|
| RT-PCR | Reverse transcription-coupled polymerase chain reaction |
| RT-PCR | Reverse transcriptional-polymerase chain reaction |
| T7 RNAP | T7 RNA polymerase |
| DOP-PCR | degenerate oligonucleotide primed polymerase chain reaction |
| DNA topoisomerase (ATP-hydrolysing) | <enzyme> An ATP-requiring enzyme which, in the presence of magnesium ions, introduces negative supertwists into closed and possibly linear duplex DNA. The enzyme is implicated in DNA replication and transcription. It causes the storage of mechanical strain energy in the superhelical turns of DNA at the expense of ATP hydrolysis. Chemical name: DNA topoisomerase (ATP-hydrolysing) Registry number: EC 5.99.1.3 (12 Dec 1998) |
|---|---|
| DNA transfection | <molecular biology> A technique originally developed to allow viral infection of animal cells by uptake of purified viral DNA rather than by intact virus particles. Term is now generally used to describe applications of same methodology to introduction of other kinds of genes or gene fragments into cells as DNA, such as activated oncogenes from tumours into tissue culture cells. (18 Nov 1997) |
| DNA tumour virus | <oncology, virology> Virus with DNA genome that can cause tumours in animals. Examples are Papovaviridae, Adenoviridae and Epstein Barr virus. (18 Nov 1997) |
| DNA tumour viruses | DNA viruses producing malignant tumours. Of the six major groupings of DNA viruses four contain members which are actually or potentially oncogenic: the adenoviridae, the herpesviridae, the papovaviridae, and the poxviridae. (12 Dec 1998) |
| DNA twisting | <molecular biology> The coiling of a double-stranded DNA molecule in the opposite direction than the direction that the helix turns. (09 Oct 1997) |
| DNA typing | <molecular biology> See restriction fragment length polymorphism. (18 Nov 1997) |
| DNA unwinding protein | <molecular biology> A protein that will attach to single-stranded DNA after it has been unwound for replication or recombination, and help it stay unwound. (09 Oct 1997) |
| DNA, viral | Deoxyribonucleic acid that makes up the genetic material of viruses. (12 Dec 1998) |
| DNA virus | <molecular biology, virology> A virus in which the nucleic acid is double or single stranded DNA (rather than RNA). Major groups of double stranded DNA viruses are papovaviruses, adenoviruses, herpes viruses, large bacteriophages and poxviruses: of single stranded, parvoviruses and coliphages _X174 and M13. (18 Nov 1997) |
| DNA viruses | Viruses whose nucleic acid is DNA. (12 Dec 1998) |
| DNA writhing | <molecular biology> Coiling of a double-stranded DNA molecule in such a way that it crosses its own (longitudinal) axis. (09 Oct 1997) |
| double-stranded complimentary DNA | <molecular biology> A duplex DNA molecule copied from a copy DNA template. (09 Oct 1997) |
| dyad symmetry of DNA | <molecular biology> Two areas of a DNA molecule whose base pair sequences are repeats of each other, inverted relative to each other, or are palindromes. (09 Oct 1997) |
| O(2)-methylthymine-DNA glycosidase | <enzyme> Repairs o(2)-methylthymidine residues in DNA Registry number: EC 3.2.2.- Synonym: mth-DNA glycosidase (26 Jun 1999) |
| O(4)-methylthymine-DNA methyltransferase | <enzyme> Repairs o(4)-methylthymidine residues in DNA Registry number: EC 2.1.1.- Synonym: mth-DNA methyltransferase (26 Jun 1999) |