| cooled-knife method | The cutting of frozen sections with a knife cooled to a few degrees below the freezing point. (05 Mar 2000) |
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| copper sulfate method | A method for the determination of specific gravity of blood or plasma in which the blood or plasma is delivered by drops into solutions of copper sulfate graded in specific gravity by increments of 0.004, each of the bottles of solution being within the expected range of the blood or plasma sample; the specific gravity of the copper sulfate solution in which the drop of blood or plasma remains suspended indefinitely indicates the specific gravity of the sample. (05 Mar 2000) |
| Politzer method | Inflation of the eustachian tube and tympanum by forcing air into the nasal cavity at the instant the patient swallows. (05 Mar 2000) |
| correlational method | A statistical method, most often used in clinical and other applied areas of psychology, to study the relationship which exists between one characteristic and another in an individual. (05 Mar 2000) |
| Porges method | A method of destroying the capsule of bacteria by heating with N/4 hydrochloric acid and neutralizing with NaOH. (05 Mar 2000) |
| cross-sectional method | <epidemiology> The study of the life span involving comparison of groups of individuals at different age levels. Compare: longitudinal method. (05 Mar 2000) |
| Hamilton-Stewart method | Formula to calculate cardiac output after intravenous indicator dye injection; blood flow in liters per minute is given by dividing the amount of injectant in milligrams by the product of the average dye concentration in the initial curve of the dye concentration sampled at a given point in the circulation and multiplied by the dose of dye (in milligrams) to write the curve from appearance to disappearance (in the absence of any recirculation). Synonym: Hamilton-Stewart formula, Stewart-Hamilton method. (05 Mar 2000) |
| Hammerschlag's method | A hydrometric method of determining the specific gravity of the blood by allowing a drop of blood to fall into each of a series of tubes containing mixtures of chloroform and benzene of known graded specific gravities; the specific gravity of that mixture in which the drop remains exactly suspended, neither rising nor falling, corresponds to the specific gravity of the blood sample. (05 Mar 2000) |
| Sanger Coulson method | The most popular method of DNA sequence determination (c.f. Maxam Gilbert sequencing). Starting with single stranded template DNA, a short complementary primer is annealed and extended by a DNA polymerase. The reaction is split into 4 tubes (called A, C, G or T) each containing a low concentration of the indicated dideoxy nucleotide, in addition to the normal deoxynucleotides. Dideoxynucleotides, once incorporated, block further chain extension and so each tube accumulates a mixture of chains of lengths determined by the template sequence. The 4 reactions are denatured and run out on an acrylamide sequencing gel in neighbouring lanes and the sequence read up the gel according to the order of the bands. (18 Nov 1997) |
| Sanger method | The method for the sequencing of DNA employing an enzyme that can polymerase DNA and labelled nucleotides. (05 Mar 2000) |
| Scarpa's method | Cure of aneurysm by ligation of the artery at some distance above the sac. (05 Mar 2000) |
| Schafer's method | An obsolete method of resuscitation in cases of drowning or asphyxia; the patient is laid face downward and natural breathing is imitated by gentle intermittent pressure over the lower part of the thorax at the rate of about 15 times a minute. (05 Mar 2000) |
| Schede's method | Filling of the defect in bone, after removal of a sequestrum or scraping away carious material, by allowing the cavity to fill with blood which may become organised (Schede's clot). (05 Mar 2000) |
| Schick method | A test for susceptibility to Corynebacterium diphtheriae toxin: 0.1 ml of Schick test toxin is injected into the skin of one forearm (test site) and the same quantity of the same, but heat-inactivated, material into the skin of the other forearm (control site); individuals with toxin-neutralizing antibodies either will have no reaction at either injection site (negative test) or may have a pseudoreaction due to antibodies for substances (antigens) in the test materials other than diphtheria toxin; individuals lacking toxin-neutralizing antibodies may have a positive reaction, which consists of an area of redness appearing 24 to 36 hours at the test site only and persisting for 4 to 5 days. Synonym: Schick method. (05 Mar 2000) |
| schlieren method | <radiobiology> An optical technique that detects density gradients occuring in a fluid flow. In its simplest form, light from a slit is collimated by a lens and focused onto a knife edge by a second lens, the flow pattern is placed between the two lenses, and the resulting diffraction pattern is observed on a screen or photographic film placed behind the knife edge. (09 Oct 1997) |