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urate crystals stain <technique> A stain using silver methenamine to detect crystals, which polarise light in contrast with calcium crystals; useful in diagnosing gout and kidney infarcts resulting from uric acid build-up.
(05 Mar 2000)
Kossa stain <technique> A stain for calcium in mineralised tissue, utilizing a silver nitrate solution followed by sodium thiosulfate; calcified bone but not osteoid is stained brown to black.
Synonym: Kossa stain.
(05 Mar 2000)
Kronecker's stain <technique> A 5% sodium chloride stain rendered faintly alkaline with sodium carbonate, used in the examination of fresh tissues under the microscope.
(05 Mar 2000)
Feulgen stain <technique> A selective cytochemical reaction for DNA in which sections or cells are first hydrolyzed with hydrochloric acid to produce apurinic acid and then are stained with Schiff's reagent to produce magenta-stained nuclei; generally the concentration of DNA in nucleoli and mitochondria is too low to permit detection by this stain
See: Kasten's fluorescent Feulgen stain.
(05 Mar 2000)
Field's rapid stain <technique> A stain to permit rapid positive diagnosis of malaria in endemic areas by using thick films; it employs methylene blue and azure B in a phosphate buffer, with the preparation counterstained by eosin in a phosphate buffer.
(05 Mar 2000)
Fink-Heimer stain <technique> A method used for histologic demonstration of degenerating nerve fibres and terminals of the central nervous system (black on a yellow background).
(05 Mar 2000)
Flemming's triple stain <technique> A stain comprised of safranin, methyl violet, and orange G.
(05 Mar 2000)
fluorescence plus Giemsa stain <technique> A stain used to demonstrate sister chromatid exchange; cells are grown in 5-bromodeoxyuridine, followed by chromosome preparation, staining in Hoechst 33258, exposure to light, and staining in Giemsa; chromosomes exhibit a "harlequin" appearance.
(05 Mar 2000)
fluorescent stain <technique> A stain or staining procedure using a fluorescent dye or substance that will combine selectively with certain tissue components and that will then fluoresce upon irradiation with ultraviolet or violet-blue light.
(05 Mar 2000)
Fontana-Masson silver stain <technique> A stain used to demonstrate melanin and argentaffin granules.
Synonym: Fontana-Masson silver stain.
(05 Mar 2000)
Fontana's stain <technique> A traditional method for silver-impregnation of treponemes and other spirochetal forms.
(05 Mar 2000)
Foot's reticulin impregnation stain <technique> A silver stain in which reticulin stains black and collagen stains golden brown; sections are floated on the surface of solutions to avoid contamination with silver debris.
(05 Mar 2000)
Laquer's stain <technique> For alcoholic hyalin, a combination of Altmann's aniline-acid fuchsin stain with a Masson trichrome stain which, on a gray-brown background, stains alcoholic hyalin red, collagen green, and nuclei brown.
(05 Mar 2000)
Fouchet's stain <technique> Fouchet's reagent employed to demonstrate bile pigments; paraffin sections are used for conjugated bile pigments, frozen sections for unconjugated ones.
(05 Mar 2000)
Fraser-Lendrum stain <technique> For fibrin, a multistaining procedure after Zenker's fixative in which fibrin, keratin, and some cytoplasmic granules appear red, erythrocytes appear orange, and collagen appears green.
(05 Mar 2000)
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