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Ziehl-Neelsen stain <technique> A method for staining acid-fast bacteria using Ziehl's stain, decolorizing in acid alcohol, and counterstaining with methylene blue; acid-fast organisms appear red, other tissue elements light blue; a modification of this stain is also used for Actinomycetes and Brucella.
(05 Mar 2000)
Ziehl's stain <technique> A carbol-fuchsin solution of phenol and basic fuchsin used to demonstrate bacteria and cell nuclei.
(05 Mar 2000)
Kinyoun stain <technique> A method for demonstrating acid-fast microorganisms, using carbol fuchsin, acid alcohol, and methylene blue; acid-fast microorganisms appear red against a blue background.
(05 Mar 2000)
Unna-Pappenheim stain <technique> A contrast stain consisting of a methyl green-pyronin solution; originally used for gonococci, but later used to detect RNA and DNA in tissue sections; RNA is stained red and DNA appears green; used to demonstrate plasma cells during chronic inflammation.
See: methyl green-pyronin stain.
(05 Mar 2000)
Unna's stain <technique> An alkaline methylene blue stain for plasma cells, a polychrome methylene blue stain with which mast cells are stained red (metachromatic).
(05 Mar 2000)
Unna-Taenzer stain <technique> An orcein solution used for staining elastic tissue.
Synonym: Unna-Taenzer stain.
(05 Mar 2000)
Kleihauer's stain <technique> A combination of aniline blue and Biebrich scarlet red used for detection of foetal cells in the maternal blood.
(05 Mar 2000)
Klinger-Ludwig acid-thionin stain <technique> For sex chromatin, a method using a preliminary acid treatment on buccal smears, prior to staining with buffered thionin, to differentiate Barr body.
(05 Mar 2000)
Kluver-Barrera Luxol fast blue stain <technique> In combination with cresyl violet, a stain useful for demonstrating myelin and Nissl substance.
(05 Mar 2000)
uranyl acetate stain <technique> A stain used in electron microscopy; uranyl acetate binds specifically to nucleic acids but selectively tends to be abolished by osmium fixation; proteins are well stained, but cytomembranes are poorly stained.
(05 Mar 2000)
urate crystals stain <technique> A stain using silver methenamine to detect crystals, which polarise light in contrast with calcium crystals; useful in diagnosing gout and kidney infarcts resulting from uric acid build-up.
(05 Mar 2000)
Kossa stain <technique> A stain for calcium in mineralised tissue, utilizing a silver nitrate solution followed by sodium thiosulfate; calcified bone but not osteoid is stained brown to black.
Synonym: Kossa stain.
(05 Mar 2000)
Kronecker's stain <technique> A 5% sodium chloride stain rendered faintly alkaline with sodium carbonate, used in the examination of fresh tissues under the microscope.
(05 Mar 2000)
Feulgen stain <technique> A selective cytochemical reaction for DNA in which sections or cells are first hydrolyzed with hydrochloric acid to produce apurinic acid and then are stained with Schiff's reagent to produce magenta-stained nuclei; generally the concentration of DNA in nucleoli and mitochondria is too low to permit detection by this stain
See: Kasten's fluorescent Feulgen stain.
(05 Mar 2000)
Field's rapid stain <technique> A stain to permit rapid positive diagnosis of malaria in endemic areas by using thick films; it employs methylene blue and azure B in a phosphate buffer, with the preparation counterstained by eosin in a phosphate buffer.
(05 Mar 2000)
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