| selfish DNA | <molecular biology> DNA that seemingly exists only for the sake of existing and is never expressed in the phenotype. (09 Oct 1997) |
|---|---|
| sequence analysis, DNA | A multistage process that includes DNA cloning, physical mapping, subcloning, sequencing, and information analysis. New technological advances have led to the automation of certain steps in this process and contribute to the potential for large-scale DNA sequencing efforts. (12 Dec 1998) |
| Pyrococcus sp GB-D DNA polymerase | <enzyme> Thermophilic enzyme from the archaeon pyrococcus; used in pcr site-directed mutagenesis Registry number: EC 2.7.7.- Synonym: gb-d polymerase (26 Jun 1999) |
| hybrid DNA | <molecular biology> A double-stranded DNA molecule which was made by hybridising two single-stranded DNA molecules from two different sources. If the two single-stranded DNA molecules have enough nucleotide sequences in common, they are able to form hydrogen bonds to each other's common sequences. (09 Oct 1997) |
| hydroxymethyluracil DNA glycosylase | <enzyme> Functions to eliminate hydroxymethyluracil from oxidatively damaged DNA Registry number: EC 3.2.2.- Synonym: hmu DNA glycosylase (26 Jun 1999) |
| site-specific DNA-methyltransferase (adenine-specific) | <enzyme> An enzyme responsible for producing a species-characteristic methylation pattern on adenine residues in a specific short base sequence in the host cell DNA. The enzyme catalyses the methylation of DNA adenine in the presence of s-adenosyl-l-methionine to form DNA containing 6-methylaminopurine and s-adenosyl-l-homocysteine. Registry number: EC 2.1.1.72 (12 Dec 1998) |
| site-specific DNA methyltransferase (cytosine-specific) | <enzyme> An enzyme responsible for producing a species-characteristic methylation pattern on cytosine residues in a specific short base sequence in the host cell's DNA. The enzyme catalyses the methylation of DNA cytosine in the presence of s-adenosyl-l-methionine to form s-adenosyl-l-homocysteine and DNA containing 5-methylcytosine. Registry number: EC 2.1.1.73 (12 Dec 1998) |
| spacer DNA | <molecular biology> The DNA sequence between genes. In bacteria, only a few nucleotides long. In eukaryotes, can be extensive and include repetitive DNA, comprising the majority of the DNA of the genome. The term is used particularly for the spacer DNA between the many tandemly repeated copies of the ribosomal RNA genes. (18 Nov 1997) |
| sticky-ended DNA | Double-stranded DNA in which one of the strands protrudes from the other strand (i.e., has a number of unpaired bases) at one end or more. (05 Mar 2000) |
| noncoding DNA | <molecular biology> DNA that does not code for part of a polypeptide chain or RNA.This includes introns and pseudo genes. In eukaryotes the majority of the DNA is noncoding. Noncoding strand refers to the so called nonsense strand, as opposed to the sense strand which is actually translated into mRNA. (18 Nov 1997) |
| nucleoskeletal DNA | <molecular biology> DNA that is proposed to exist mostly to maintain nuclear volume and not for coding protein. (18 Nov 1997) |
| superhelical DNA endonuclease | <enzyme> Produces single nicks in superhelical DNA; co-purifies with calf thymus terminal deoxynucleotidyl transferase Registry number: EC 3.1.21.- (26 Jun 1999) |
| Deep Vent DNA polymerase | <enzyme> A thermostable DNA polymerase with putative proofreading activity Registry number: EC 2.7.7.- (26 Jun 1999) |
| denaturation temperature of DNA | That temperature at which, under a given set of conditions, double-stranded DNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., GC content) of the DNA. Synonym: melting temperature of DNA. (05 Mar 2000) |
| DNA | <cell biology, molecular biology> Deoxyribonucleic acid. The molecule that encodes genetic information in the nucleus of cells. It determines the structure, function and behaviour of the cell. DNA is a double-stranded molecule held together by weak bonds between base pairs of nucleotides. The four nucleotides in DNA contain the bases: adenine (A), guanine (G), cytosine (C), and thymine (T). In nature, base pairs form only between A and T and between G and C, thus the base sequence of each single strand can be deduced from that of its partner. (10 Nov 1998) |