| centrifugation, density gradient | Separation of particles according to density by employing a gradient of varying densities. at equilibrium each particle settles in the gradient at a point equal to its density. (12 Dec 1998) |
|---|---|
| cesium chloride gradient centrifugation | A type of density gradient centrifugation, a lab technique used to separate or purify nucleic acids. It involves putting cesium chloride and the nucleic acids into a centrifuge to be spun for hours or days. The cesium chloride forms a density gradient (highly dense at the bottom, thinnest at the top), and the different nucleic acids separate along the gradient according to their buoyancies in different densities. (09 Oct 1997) |
| density gradient centrifugation | <technique> High-speed centrifugation inwhich molecules float at a point wheretheir density equals that in a gradient of cesium chloride or sucrose. (12 Jan 1998) |
| sucrose | Nonreducing disaccharide, _ D glucopyranosyl _ D fructofuranose. (18 Nov 1997) |
| sucrose alpha-d-glucohydrolase | <enzyme> An enzyme hydrolyzing sucrose and maltose; in a complex with isomaltase; hence, hydrolyzes both sucrose and isomaltose; found in the intestinal mucosa; a deficiency of this enzyme results in defective digestion of sucrose and linear a1,4-glucans. Synonym: sucrase. (05 Mar 2000) |
| sucrose - fructan 6-fructosyltransferase | <enzyme> Catalyses the formation and extension of beta-2,6-linked fructans, typical of grasses; from barley, hordeum vulgare; genbank x83233 Registry number: EC 2.4.1.- Synonym: barley 6-sft (26 Jun 1999) |
| sucrose haemolysis test | Isotonic sucrose promotes binding of complement to red blood cells; in paroxysmal nocturnal haemoglobinuria a proportion of the cells is sensitive to complement-mediated lysis, and haemolysis ensues. (05 Mar 2000) |
| sucrose permease | <chemical> Sucrose-utilizing enzyme Chemical name: permease, sucrose (26 Jun 1999) |
| sucrose-phosphate synthase phosphatase | <enzyme> A type 2a phosphoprotein phosphatase Registry number: EC 3.1.3.- Synonym: sps-phosphatase (26 Jun 1999) |
| sucrose synthase | <enzyme> Sus1 gene product isolated from maise Registry number: EC 2.4.1.13 Synonym: sucrose synthetase, udp-glucose-d-fructose-2-glycosyltransferase, suc synthase 1, suc synthase1, sus1 gene product (26 Jun 1999) |
| dietary sucrose | Sucrose present in the diet. It is added to food and drinks as a sweetener. (12 Dec 1998) |
| band centrifugation | <technique> High-speed centrifugation inwhich molecules float at a point wheretheir density equals that in a gradient of cesium chloride or sucrose. (12 Jan 1998) |
| centrifugation | <procedure> The process of separating fractions of systems in a centrifuge. The most basic separation is to sediment a pellet at the bottom of the tube, leaving a supernatant at a given centrifugal force. In this case sedimentation is determined by size and density of the particles in the system amongst other factors. Density may be used as a basis for sedimentation in density gradient centrifugation. at very high g values molecules may be separated, i.e. Ultracentrifugation. In continuous centrifugation the supernatant is removed continuously as it is formed. (13 Nov 1997) |
| centrifugation, isopycnic | Centrifugation in which the solvent is of the same density as the substance to be isolated. From the greek iso-, equal and pyknos, thick. (12 Dec 1998) |
| centrifugation, zonal | Centrifugation using a rotating chamber of large capacity in which to separate cell organelles by density-gradient centrifugation. (12 Dec 1998) |