| SCPNT | Southern California Postrotary Nystagmus Test |
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| SCSIT | Southern California Sensory Integration Test |
| STSA | Southern Thoracic Surgical Association |
| SB | Southern blotting |
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| WB | Westen blotting |
| WLB | Western Ligand Blotting |
| immunoblotting | blotting |
| ENSO | El Nino Southern Oscillation |
| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
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| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
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| blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
| blotting, northern | Detection of RNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, western | Identification of proteins or peptides that have been electrophoretically separated by blotting and transferred to strips of nitrocellulose paper. The blots are then detected by radiolabelled antibody probes. (12 Dec 1998) |
| Western blotting | <molecular biology> An electroblotting method in which proteins are transferred from a gel to a thin, rigid support (nitrocellulose) and detected by binding of labelled antibody. See: blots. (18 Nov 1997) |
| africa, southern | The geographical area of africa comprising angola, botswana, lesotho, malawi, mozambique, namibia, south africa, swaziland, zambia, and zimbabwe. It includes what was formerly called south-west africa or german southwest africa but it was terminated in 1966 by a united nations resolution. (12 Dec 1998) |
| blot, southern | A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot. (12 Dec 1998) |
| Southern | M.E., 20th century British biologist. See: Southern blot analysis. (05 Mar 2000) |
| southern blot | A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997) |
| Southern blot analysis | A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes. (05 Mar 2000) |
| Southern blotting method |
A technique used in molecular genetics to analyze a small portion of DNA first by purifying it, then by controlled fragmentation, electrophoretic separation, and fixing the fragment identity using specific DNA probes. It
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