| recombinant DNA | <molecular biology> Spliced DNA formed from two or more different sources that have been cleaved by restriction enzymes and joined by ligases. (18 Nov 1997) |
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| recombinant DNA molecules | A combination of DNA molecules of different origin that are joined using recombinant DNA technology. (12 Dec 1998) |
| recombinant DNA technologies | Procedures used to join together DNA segments in a cell-free system (an environment outside a cell ororganism). Under appropriate conditions, a recombinant DNA molecule canenter a cell and replicate there, either autonomously or after it hasbecome integrated into a cellular chromosome. (09 Oct 1997) |
| recombinant DNA technology | A series of procedures used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed (recombined) from segments from 2 or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, autonomously (on its own) or after it has become integrated into a chromosome. (12 Dec 1998) |
| molecules, recombinant DNA | A combination of DNA molecules of different origin that are joined using recombinant DNA technology. (12 Dec 1998) |
| DNA molecules, recombinant | A combination of DNA molecules of different origin that are joined using recombinant DNA technology. (12 Dec 1998) |
| DNA, recombinant | Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. (12 Dec 1998) |
| DNA technology, recombinant | A series of procedures used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed (recombined) from segments from 2 or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, autonomously (on its own) or after it has become integrated into a chromosome. (12 Dec 1998) |
| technology, recombinant DNA | A series of procedures used to join together (recombine) DNA segments. A recombinant DNA molecule is constructed (recombined) from segments from 2 or more different DNA molecules. Under certain conditions, a recombinant DNA molecule can enter a cell and replicate there, autonomously (on its own) or after it has become integrated into a chromosome. (12 Dec 1998) |
| random amplified polymorphic DNA technique | Technique that utilises low-stringency polymerase chain reaction (pcr) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. Rapd technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. (12 Dec 1998) |
| DNA-directed DNA polymerase | <enzyme> DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair. Chemical name: Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed) Registry number: EC 2.7.7.7 (12 Dec 1998) |
| recombinant | <molecular biology> A cell or an individual with a new combination of genes not found together in either parent, usually applied to linked genes. (18 Nov 1997) |
| recombinant clone | <molecular biology> Clones containing recombinant DNA molecules. See: recombinant DNA technologies. (14 Oct 1997) |
| recombinant clones | Clones containing recombinant DNA molecules. (12 Dec 1998) |
| recombinant fusion proteins | Proteins that are the result of genetic engineering. A regulatory part or promoter of one or more genes is combined with a structural gene. The fusion protein is formed after transcription and translation of the fused gene. This type of fusion protein is used in the study of gene regulation or structure-activity relationships. They might also be used clinically as targeted toxins (immunotoxins). (12 Dec 1998) |