| methyl green-pyronin stain | <technique> A staining method useful for identification of plasma cells which are intensely pyroninophilic; a mixture of a green and a red dye that has the property of staining highly polymerised nucleic acid (DNA) green and low molecular weight nucleic acids (RNA) red. See: Unna-Pappenheim stain. (05 Mar 2000) |
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| pyronin | A fluorescent red basic xanthene dye, the chloride of tetramethyldiaminoxanthene, pyronin Y or pyronin G, or of tetraethyldiaminoxanthene pyronin B. These dyes, especially pyronin Y, are used in combination with methyl green for differential staining of RNA (red) and DNA (green); difference in staining result is probably due to the higher degree of polymerization of DNA; pyronin Y is also used as a tracking dye for RNA in electrophoresis. (05 Mar 2000) |