| SDS-PAGE | Sodium DodecylSulfate-PolyacrylAmide Gel Electrophoresis |
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| H-PAGE | horizontal polyacrylamide gel |
| PAG | periaqueductal gray [matter]; polyacrylamide gel; pregnancy-associated globulin; proliferation-assoc... |
| PAGE | polyacrylamide gel electrophoresis |
| PAGIF | polyacrylamide gel isoelectric focusing |
| BN-PAGE | Blue Native polyacrylamide gel electrophoresis |
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| PAGE | Polyacrylamide gel electrophoretic |
| PAGIEF | Polyacrylamide gel isoelectric focusing |
| PAG | polyacrylamide gel |
| PAGE | SDS)--polyacrylamide gel electrophoresis |
| polyacrylamide gel electrophoresis | Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the electrophoresis takes place and contributes molecular sieving. Commonly carried out in the presence of the anionic detergent sodium dodecylsulphate (SDS). SDS denatures proteins so that noncovalently associating sub unit polypeptides migrate independently and by binding to the proteins confers a net negative charge roughly proportional to the chain weight. See: SDS PAGE. (21 Jun 1999) |
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| electrophoresis, polyacrylamide gel | Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (12 Dec 1998) |
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| polyacrylamide | A branched polymer of acrylamide (H2C==CHCONH2) that is used in gel electrophoresis; e.g., R-CH2-CH(CONH2)-CH(CONHRCH(CONHR')-R'. (05 Mar 2000) |
| agar-gel reaction | <immunology> The reaction between an antibody and an antigen during an immunology lab procedure where the two are allowed to diffuse toward each other through an agar-gel medium. Lines of precipitation form in the places on the gel where the two react with each other and shows where the reaction has occurred. (09 Oct 1997) |
| agarose gel electrophoresis | <procedure> A type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size. (06 May 1997) |
| aluminum hydroxide gel | A suspension containing Al2O3, mainly in the form of aluminum hydroxide, used as an antacid; a dried form, with the same use, is obtained by drying the product of interaction in aqueous solution of an aluminum salt with ammonium or sodium carbonate. (05 Mar 2000) |
| aluminum phosphate gel | An aqueous suspension of between 4.0 and 5.0% of aluminum phosphate; used as an antacid. (05 Mar 2000) |
| bag-gel implant | An implant composed of a silicone rubber bag containing a silicone gel; used in augmentation mammaplasty. (05 Mar 2000) |
| gel | Jelly like material formed by the coagulation of a colloidal liquid. Many gels have a fibrous matrix and fluid filled interstices: gels are viscoelastic rather than simply viscous and can resist some mechanical stress without deformation. Examples are the gels formed by large molecules such as collagen (and gelatin), agarose, acrylamide and starch. (18 Nov 1997) |
| gel diffusion | Diffusion in a gel, as in the case of gel diffusion precipitin tests in which the immune reactants diffuse in agar. See: immunodiffusion. (05 Mar 2000) |
| gel diffusion precipitin tests | Precipitin test's in which the immune precipitate forms in a gel medium (usually agar) into which one or both reactants have diffused; generally classified in two types, in one dimension, and in two dimensions. Synonym: gel diffusion reactions. (05 Mar 2000) |
| gel diffusion precipitin tests in one dimension | Precipitin test's in which antigen solution and antibody incorporated in agar are layered in tubes, permitting effective diffusion in the vertical dimension; the antibody-containing agar may be overlaid directly with antigen solution (single (gel) diffusion in one dimension). (05 Mar 2000) |
| gel diffusion precipitin tests in two dimensions | Precipitin test's made in a layer of agar that permits radial diffusion, in both of the horizontal dimensions, of one or both reactants. Double (gel) diffusion in two dimensions (Ouchterlony test, technique, or method) incorporates antigen and antibody solutions placed in separate wells in a sheet of plain agar, permitting radial diffusion of both reactants; this method is widely used to determine antigenic relationships; the bands of precipitate that form where the reactants meet in optimal concentration are of three patterns, referred to as reaction of identity, reaction of partial identity (cross-reaction), and reaction of nonidentity. (05 Mar 2000) |
| gel diffusion reactions | Precipitin test's in which the immune precipitate forms in a gel medium (usually agar) into which one or both reactants have diffused; generally classified in two types, in one dimension, and in two dimensions. Synonym: gel diffusion reactions. (05 Mar 2000) |
| gel electrophoresis | <molecular biology> Electrophoresis using a gel supporting phase. Usually applied to systems where the gel is based on polyacrylamide. See: electrophoresis. (05 May 1997) |
| gel exclusion chromatography | <investigation> A lab technique, a type of column chromatography, used to separate the components of a mixture by molecular size and to collect the molecules which are larger than a certain size. It is similar to gel filtration, small molecules are slowed or trapped by the pores in the gel beads filling the column, while large molecules, too large to fit into the pores, slide past the beads and get to the bottom of the column first. at this point, the large molecules are collected. Gel exclusion refers to the maximum size of molecule which will fit into the gel bead pores, and this lab technique is used to collect the molecules in the mixture which are larger than, or excluded from, the pores. (09 Oct 1997) |
| polyacrylamide gel |
A matrix of acrylamide polymers cross-linked using bis-acrylamide. Polyacrylamide gels are used for the separation of nucleic acids and proteins by gel electrophoresis.
Ãâó: www.genpromag.com/Glossary~LETTER~P.html
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| polyacrylamide gel electrophoresis |
Electrophoresis of nucleic acids and proteins through polyacrylamide gels.
Ãâó: www.genpromag.com/Glossary~LETTER~P.html
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| polyacrylamide gel e. |
gel electrophoresis using a polymerized polyacrylamide matrix to separate molecules on the basis of size, charge, or both; usually used to separate proteins or sequence nucleic acids. Gels are usually discontinuous (see disc e.) but may be a single layer, and are either nondenaturing to examine native molecules or denaturing as in SDS-PAGE.
Ãâó: www.mercksource.com/pp/us/cns/cns_health_library.j...
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| polyacrylamide gel electrophoresis |
One of the two major types of gels used for the electrophoretic separation of DNA molecules. PAGE, in contrast to agarose gel electrophoresis, is generally used when the DNA fragments to be separated are small. When used in a sequencing gel format, it is capable of single nucleotide resolution.
Ãâó: pathology2.jhu.edu/molec/glossary.cfm
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