| replica plating | <technique> Technique for testing the genetic characteristics of bacterial colonies. A dilute suspension of bacteria is first spread, in a petri dish, on agar containing a medium expected to support the growth of all bacteria, the master plate. Each bacterial cell in the suspension is expected to give rise to a colony. A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony. The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement. The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics. Thus colonies can be identified that cannot grow without specific nutrients or that are antibiotic resistant and cells with mutations in particular genes can be isolated. (18 Nov 1997) |
|---|---|
| compression plating | A technique for internal fixation of fractures in which plates and screws are applied so as to produce compression of the line of fracture. (05 Mar 2000) |
| plating | 1. The art or process of covering anything with a plate or plates, or with metal, particularly of overlaying a base or dull metal with a thin plate of precious or bright metal, as by mechanical means or by electromagnetic deposition. 2. A thin coating of metal laid upon another metal. 3. A coating or defensive armor of metal (usually steel) plates. Source: Websters Dictionary (01 Mar 1998) |
| Abbott's method | A method of treatment of scoliosis by use of a series of plaster jackets applied after partial correction of the curvature by external force. (05 Mar 2000) |
| Abell-Kendall method | A standard method for estimation of total serum cholesterol involving saponification of cholesterol ester by hydroxide, extraction with petroleum ether, and colour development with acetic anhydride-sulfuric acid; the method avoids interference by bilirubin, protein, and haemoglobin. (05 Mar 2000) |
| activated sludge method | A method of sewage disposal in which the sewage is treated with 15% bacterially active, liquid sludge, which is produced by repeated vigorous aeration of fresh sewage to form floccules or sediment; when this flocculation process is complete, the resulting activated sludge contains large numbers of bacteria, together with yeasts, molds, and protozoa, which actively effect the oxidation of organic compounds; this mixture is piped to a sedimentation tank, the effluent from which is completely treated sewage. (05 Mar 2000) |
| Altmann-Gersh method | The method of rapidly freezing a tissue and dehydrating it in a vacuum. (05 Mar 2000) |
| Anel's method | Ligation of an artery immediately above (on the proximal side of) an aneurysm. (05 Mar 2000) |
| Antyllus' method | Ligation of the artery above and below an aneurysm, followed by incision into and emptying of the sac. (05 Mar 2000) |
| aristotelian method | A method of study that stresses the relation between a general category and a particular object. (05 Mar 2000) |
| Ashby method | A differential agglutination method for estimating erythrocyte life span; compatible blood possessing a group factor that the recipient lacks is transferred to the recipient; after the transfusion, sera with potent agglutinins for the recipient's red cells are added to samples of the recipient's blood, and the unagglutinated red cells are counted; using this technique the red cell life span in normal persons is found to be 110 to 120 days. (05 Mar 2000) |
| auxanographic method | A method for the study of bacterial enzymes in which agar is mixed with the material (e.g., starch or milk) which is to serve as an indicator of the enzyme action and is inoculated and plated; if the bacteria produce enzymes digesting the admixed material, there will be a zone of clearing in the medium about each colony. Synonym: diffusion method. (05 Mar 2000) |
| Barraquer's method | Zonulysis Dissolution of the zonula ciliaris by enzymes (alpha-chymotrypsin) to facilitate surgical removal of a cataract. Synonym: Barraquer's method. Origin: zonule + G. Lysis, dissolution (05 Mar 2000) |
| Beck's method | A permanent opening into the stomach made from its greater curvature. (05 Mar 2000) |
| Bier's method | Treatment of various surgical conditions by reactive hyperaemia. (05 Mar 2000) |