| FORTRAN | formula translation |
|---|---|
| IVTT | in vitro transcription and translation |
| TIP | thermal inactivation point; Toxicology Information Program; translation-inhibiting protein; tumor-in... |
| ISNT | In situ nick translation |
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| eIF | Eukaryotic translation initiation factor |
| eIF-2 | Eukaryotic translation initiation factor 2 |
| eIF3 | Eukaryotic translation initiation factor 3 |
| eIF-4E | Eukaryotic translation initiation factor 4E |
| nick translation | <molecular biology, technique> A technique used to radioactively label DNA. E. Coli DNA polymerase I will add a nucleotide, copying the complementary strand, to the free 3' OH group at a nick, at the same time its exonuclease activity removes the 5' terminus. The enzyme then adds a nucleotide at the new 3' OH and removes the new 5' terminus. In this way one strand of the DNA is replaced starting at a nick, which effectively moves along the strand. Nick translation refers to this translation or movement and not to protein synthesis. In practice, DNA is mixed with trace amounts of DNAase I to generate nicks, DNA polymerase I and labelled nucleotides. Because the nicks are generated randomly the DNA preparation can be uniformly labelled and to a high degree of specific activity. (10 Jan 1998) |
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| nick | <molecular biology> A point in a double stranded DNA molecule where there is no phosphodiester bond between adjacent nucleotides of one strand typically through damage or enzyme action. (18 Nov 1997) |
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| Nick's procedure | Enlarges the aortic annulus by incising the noncoronary sinus and the roof of the left atrium. (05 Mar 2000) |
| polarity of translation | Decrease in the synthesis of proteins specified by genes distal to the operator and to the site of a nonsense, frameshift, deletion or insertion mutation in an operon. A mutation which produces polarity is called a polar mutation or a polarity mutation. (12 Dec 1998) |
| hybrid-arrested translation | <molecular biology> The prevention of an mRNA molecule from being translated into a protein by hybridising it to its corresponding cDNA or to a complementary mRNA. This is used to identify cDNA molecules - the scientist puts the cDNA molecule in question in a test tube with a number of mRNA molecules and observes which protein is no longer able to be made. (09 Oct 1997) |
| translation | The process that occurs at the ribosome whereby the information in mRNA is used to specify the sequence of amino acids in a polypeptide chain. (18 Nov 1997) |
| translation, genetic | Formation of peptides on ribosomes, directed by messenger RNA. (12 Dec 1998) |
| nick translation |
A procedure for labelling DNA. A DNA fragment is treated with DNase to produce single-stranded nicks. The nick is moved along the DNA molecule in the presence of labelled deoxyribonucleoside triphosphates by the concerted action of the 5?> 3?exonuclease and 5?> 3?polymerase activities of E. coli DNA polymerase I.
Ãâó: www.fao.org/docrep/003/X3910E/X3910E17.htm
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| nick translation |
A DNA probe labeling technique based on the introduction of nucleotide gaps into one strand of double-stranded DNA by a treatment with a nuclease, followed by the use of a DNA polymerase with exonuclease activity to extend the gaps and replace the nucleot
Ãâó: www.genpromag.com/Glossary~LETTER~N.html
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| nick translation |
Use of enzymes to break DNA and repolymerize small sections of the molecule, usually for purposes of labeling the DNA with a radioactive nucleotide.
Ãâó: www.hardydiagnostics.com/Glossary-N.html
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| nick translation |
describes a probe labelling technique where DNA polymerase I nicks a starting point from which one strand of a duplex DNA can be degraded and replaced by resynthesis with new labelled precursor nucleotides.
Ãâó: www.molecularpathology.com/pt/re/dmp/fulltext.0001...
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