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isoelectric focusing Isoelectric focusing is a method of separating proteins based on their relative content of acidic and basic residues. This content is represented by a value known as an isoelectric point, or pI. Proteins are introduced into a gel (composed of polyacrylamide, starch, agarose, etc.) which has an established pH gradient or is capable of establishing such a gradient after applying an electrical current. ...
Ãâó: en.wikipedia.org/wiki/Isoelectric_focusing
isoelectric point The isoelectric point (or pI) of a protein is the pH at which the protein has an equal number of positive and negative charges.
Ãâó: www.nature.com/nrmicro/journal/v2/n6/glossary/nrmi...
isoelectric point pH at which an amphoteric molecule has a net charge equal to zero
Ãâó: www.inproteomics.com/nwgloshi.html
isoelectric showing no variation in electric potential.
Ãâó: www.mercksource.com/pp/us/cns/cns_health_library.j...
isoelectric f. electrophoresis in which the protein mixture is subjected to an electric field in a gel medium in which a pH gradient has been established; each protein then migrates until it reaches the site (or focus) at which the pH is equal to its isoelectric point. Called also electrofocusing.
Ãâó: www.mercksource.com/pp/us/cns/cns_health_library.j...
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