| insertional inactivation | The inactivation of a gene due to the insertion of exogenous genetic material into that gene. (14 Nov 1997) |
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| insertional mutagenesis | Generally, mutagenesis of DNA by the insertion of one or more bases. Specific examples: 1. Oncogenesis by insertion of a retrovirus adjacent to a cellular proto-oncogene. 2. A strategy of mutagenesis with transposons. After a round of transposition, progeny are screened by PCR, with transposon and gene specific primers, for the proximity of the transposon sequence to the gene of interest. As PCR can only produce products up to 1-2 kb, a large fraction of progeny identified as positive by PCR will have a transposon close enough to the gene to inactivate or otherwise alter its pattern of expression. (18 Nov 1997) |
| mutagenesis, insertional | Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA can be inserted into or adjacent to a cellular proto-oncogene. Insertion of the provirus can cause mutations by interrupting coding sequences or regulatory elements, or cause unregulated expression of the proto-oncogene resulting in tumour formation. (12 Dec 1998) |
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| insertional mutagenesis |
The production of a mutation by insertion of 1 or more copies of a transgene into a host genome.
Ãâó: www.kumc.edu/gec/gloss.html
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| insertional mutagenesis |
The generation of an insertion mutation by inserting one or more extra nucleotide bases into the DNA sequence.
Ãâó: dragon.zoo.utoronto.ca/~B03T0801B/glossary.html
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| insertional mutagenesis |
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene. This process may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. [MeSH]
Ãâó: www.bioon.com/book/biology/genomicglossaries/funct...
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