| ACIF | anticomplement immunofluorescence |
|---|---|
| CLIF | cloning inhibitory factor; Crithidia luciliae immunofluorescence |
| DIF | diffuse interstitial fibrosis; direct immunofluorescence; dose increase factor |
| FCI | fixed-cell immunofluorescence; food chemical intolerance |
| GIFT | gamete intrafallopian transfer; granulocyte immunofluorescence test |
| IFM | immunofluorescence microscopy |
|---|---|
| ACIF | Anticomplement immunofluorescence |
| DIF | Direct Immunofluorescence |
| DFA | Direct immunofluorescence assay |
| IF | Immunofluorescence |
| anticomplement immunofluorescence | A technique used to make certain indirect fluorescent antibody techniques more specific and sensitive. Here the fluorescent dye is conjugated to antibody directed at complement and then added to a complement-fixing complex of antigen and patient antibody. (05 Mar 2000) |
|---|---|
| avidin-biotin immunofluorescence | Holds promise for more sensitive and specific amplification of indirect fluorescent antibody procedures. Antibody to the patient's specific antibodies is labelled with biotin, a compound capable of specifically binding avidin in high concentrations. Fluorescent labelled avidin is then added and fluorescent microscopy is used to detect the presence of the complexes. (05 Mar 2000) |
| micro-immunofluorescence | Several different substrates are arranged in specific locations on a single microscope slide well allowing a rapid, simultaneous indirect fluorescent antibody on each substrate. (05 Mar 2000) |
| immunofluorescence | <technique> A test or technique in which one or other component of an immunological reaction is made fluorescent by coupling with a fluorochrome such as fluorescein, phycoerythrin or rhodamine so that the occurrence of the reaction can be detected as a fluorescing antigen-antibody complex. Used in microscopy to localise small amounts of antigen or specific antibody. (18 Nov 1997) |
| immunofluorescence method | Any method in which a fluorescent-labelled antibody is used to detect the presence or determine the location of the corresponding antigen. (05 Mar 2000) |
| indirect immunofluorescence | <procedure> A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localised, is used unlabelled and the location of the first antibody is then detected by use of a fluorescently labelled antiIgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification and a well characterised goat antirabbit IgG antibody can, for example: be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localisation of the first antibody by substituting peroxidase or gold labelled second antibody. (18 Nov 1997) |
| aperture for electron microscopy | <technique> Anode aperture: The opening in the accelerating voltage anode shield of the electron gun through which the electrons must pass to irradiate the specimen. Condenser aperture: An opening in the condenser lens controlling the number of electrons entering the lens and the angular aperture of the electron beam. The angular aperture can also be controlled by the condenser lens current. Physical objective aperture: A metallic diaphragm, with a small central hole, used to limit the cone of electrons accepted by the objective lens. This improves image-contrast since highly scattered electrons are prevented from arriving at the Gaussian image plane and therefore cannot contribute to background fog. Aplanatic. Free from spherical aberration and coma. (05 Aug 1998) |
| bright field microscopy | <technique> Optical microscopy, in which absorption to a great extent and diffraction to a minor extent give rise to the image, as opposed to phase contrast or interference methods of microscopy. (18 Nov 1997) |
| ratio imaging fluorescence microscopy | <procedure> A method of measurement of intracellular pH or intracellular calcium levels, using a fluorescent probe molecule (see fura-2), in which the two different excitation wavelengths are used and the emitted light levels compared. If emission at one wavelength is sensitive to the intracellular ion level and emission at the other wavelength is not, then standardisation for intracellular probe concentration, efficiency of light collection, inactivation of probe and thickness of cytoplasm can all be performed automatically. (17 Dec 1997) |
| reflection X-ray microscopy | <technique> A method of producing enlarged images by means of X rays. In this method the radiation is totally reflected at glancing incidence from polished concave mirrors or from the curved surfaces of single crystals by Bragg reflection. The problem of aberration corrections still limits the resolution obtainable. (05 Aug 1998) |
| video microscopy | <technique> Microscopy that takes advantage of video as an imaging, image processing, analysing, or controlling device. (05 Aug 1998) |
| phase contrast microscopy | <investigation> A simple nonquantitative form of interference micoscopy of great utility in visualising live cells. Small differences in optical path length due to differences in refractive index and thickness of structures are visualised as differences in light intensity. (18 Nov 1997) |
| microscopy | <technique> The science of the interpretive use, and applications of microscopes. (05 Aug 1998) |
| microscopy, atomic force | Microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. A microcomputer keeps track of the vertical excursions as a function of the position of the probe in the horizontal plane and presents the sample's image. (12 Dec 1998) |
| microscopy, confocal | A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible. (12 Dec 1998) |
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