| CGH | Comparative Genomic Hybridisation |
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| ISH | In Situ Hybridisation |
| ISHH | In situ hybridisation histochemistry |
| NISH | Non-isotopic in situ hybridisation |
| SBH | Southern blot hybridisation |
| hybridisation | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
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| hybridisation stringency | <molecular biology> The percentage of nucleotides which must match on two unrelated single-stranded nucleic acid molecules before they will base pair with each other to form a duplex, given a certain set of physical and chemical conditions. The hybridisation stringency is used to determine when a hybridisation probe and a target nucleic acid will come together, and can be set by the researcher by varying the conditions. In general, if the percentage of matching nucleotides is lower than 70 percent, the two single-stranded nucleic acid molecules are considered nonhomologous and any hybridisation is considered nonstringent. (13 Oct 1997) |
| colony hybridisation | <molecular biology> A genetics lab technique used to identify which colonies of bacteria on an agar plate contain a particular sequence of DNA or a particular gene. The technique involves pressing a nylon or nitrocellulose membrane onto the plate so that each colony contributes a small smudge of itself to the membrane, then treating the membrane with chemicals and heat, then washing the membrane with a labelled probe to find the specific DNA sequence. The smudges which are indicated by the probe are then compared back to the colonies on the agar plate. This technique is often used in conjunction with experiments involving the making of genomic libraries. (09 Oct 1997) |
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| competition hybridisation | <molecular biology, technique> A lab technique used to determine how similar two strands of single-stranded nucleic acids are to each other by putting them with a third strand (called a standard) and observing how well they can bond with each other to become double-stranded (how well they hybridize). (05 Jan 1998) |
| cross-hybridisation | <molecular biology> The hydrogen bonding of asingle-stranded DNA sequence that is partially but not entirely complementary to a single-stranded substrate. Often, this involves hybridising a DNA probe for a specific DNA sequence to the homologous sequences of different species. (09 Oct 1997) |
| DNA hybridisation | <molecular biology> The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. Technique in which single stranded nucleic acids are allowed to interact so that complexes or hybrids, are formed by molecules with sufficiently similar, complementary sequences. By this means the degree of sequence identity can be assessed and specific sequences detected. The hybridisation can be carried out in solution or with one component immobilised on a gel or, most commonly, nitrocellulose paper. Hybrids are detected by various means: visualisation in the electron microscope, by radioactively labelling one component and removing noncomplexed DNA or by washing or digestion with an enzyme that attacks single stranded nucleic acids and finally estimating the radioactivity bound. Hybridisations are done in all combinations: DNA DNA (DNA can be rendered single stranded by heat denaturation), DNA RNA or RNA RNA. In situ hybridisations involve hybridising a labelled nucleic acid (often labelled with a fluorescent dye) to suitably prepared cells or histological sections. This is used particularly to look for specific transcription or localisation of genes to specific chromosomes (FISH analysis). <zoology> The mating of individuals from different species or sub-species. (13 Oct 1997) |
| DNA-RNA hybridisation | <molecular biology> A type of hybridisation. In this case, a strand of DNA is joined with a complementary strand of RNA to form a double-stranded molecule (or one which is partly double-stranded, if one of the original single strands is shorter than the other). (09 Oct 1997) |
| in situ hybridisation | <molecular biology, technique> Use of a DNA or RNA probe todetect the presence of the complementaryDNA sequence in cloned bacterial or cultured eukaryotic cells.Also used for locating geneson chromosomes. The process is: Prepare microscope slide with cells in metaphase of mitosis, Treat slide with a weak base. Thus denaturing the DNA. Pour radioactively labelled probe onto the slide. Expose slide to photographic emulsion for a few days or weeks. Develop emulsion. (13 Oct 1997) |
| hybridisation | the act of mixing different breeds of animals |
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제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
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제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|