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  • freeze-dryer
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  • freeze-drying
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  • freeze-etching
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  • freeze-fracturing
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    µ¿°á°ÇÁ¶(¹ý)(ÔÐÌ¿ËëðÏÛö).
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  • freeze-clamp technique
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  • freeze-cleaving
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  • freeze-drying
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  • freeze-etching
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  • freeze-fracturing
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  • freeze-stop technique
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  • freeze-substitution
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  • freeze-thawing
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  • freeze
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AFD accelerated freeze drying; acrofacial dysostosis
FD familial dysautonomia; family doctor; fan douche; fatal dose; fetal danger; fibrin derivative; fibro...
QF quality factor; query fever; quick freeze; relative biological effectiveness
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TEV Tobacco etch virus
TEV tobacco etch potyvirus
DFDBA DEMINERALIZED freeze-dried bone allograft
FD Freeze drying
FT Freeze-thaw
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  • freeze drying
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  • freeze-fracturing
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freeze etch <microscopy, technique> A method of preparing a cell for study by freezing it, cracking it open to reveal the organelles, freeze drying it, then examining it under the electron microscope.
(09 Oct 1997)
freeze etching If a freeze fractured specimen is left for any length of time before shadowing, then water will sublime off from the specimen etching (lowering) those surfaces that are not protected by a lipid bilayer. Some etching will take place following any freeze cleavage process, in deep etching the ice surface is substantially lowered to reveal considerable detail of, for example: cytoplasmic filament systems.
(18 Nov 1997)
CancerWEB ¿µ¿µ ÀÇÇлçÀü À¯»ç °Ë»ö °á°ú : 9 ÆäÀÌÁö: 1
acid etch <dentistry> A procedure where a weak acid smeared on your teeth to ready your teeth for brackets. The acid etch helps your brackets stay on better.
(08 Jan 1998)
acid etch cemented splint A splint of heavy wire which is cemented to the labial surfaces of teeth with any of the acid etch cement techniques; used to stabilise traumatically displaced or periodontally diseased teeth.
(05 Mar 2000)
etch 1. To produce, as figures or designs, on mental, glass, or the like, by means of lines or strokes eaten in or corroded by means of some strong acid.
The plate is first covered with varnish, or some other ground capable of resisting the acid, and this is then scored or scratched with a needle, or similar instrument, so as to form the drawing; the plate is then covered with acid, which corrodes the metal in the lines thus laid bare.
2. To subject to etching; to draw upon and bite with acid, as a plate of metal. "I was etching a plate at the beginning of 1875." (Hamerton)
3. To sketch; to delineate. "There are many empty terms to be found in some learned writes, to which they had recourse to etch out their system." (Locke)
Origin: D. Etsen, G. Atzen to feed, corrode, etch. MHG. Etzen, causative of ezzen to eat, G. Essen . See Eat.
Source: Websters Dictionary
(01 Mar 1998)
freeze 1. To become congealed by cold; to be changed from a liquid to a solid state by the abstraction of heat; to be hardened into ice or a like solid body.
Water freezes at 32 deg above zero by Fahrenheit's thermometer; mercury freezes at 40 deg below zero.
2. To become chilled with cold, or as with cold; to suffer loss of animation or life by lack of heat; as, the blood freezes in the veins. To freeze up (
Origin: OE. Fresen, freosen, AS. Freosan; akin to D. Vriezen, OHG. Iosan, G. Frieren, Icel. Frjsa, Sw. Frysa, Dan. Fryse, Goth. Frius cold, frost, and prob. To L. Prurire to itch, E. Prurient, cf. L. Prna a burning coal, pruina hoarfrost, Skr. Prushva ice, prush to spirt. 18. Cf. Frost.
Source: Websters Dictionary
(01 Mar 1998)
freeze cleavage Method of specimen preparation for the electron microscope in which rapidly frozen tissue is cracked so as to produce a fracture plane through the specimen. The surface of the fracture plane is then shadowed by heavy metal vapour, strengthened by a carbon film and the underlying specimen is digested away, leaving a replica that can be picked up on a grid and examined in the transmission electron microscope. The great advantage of the method is that the fracture plane tends to pass along the centre of lipid bilayers and it is therefore possible to get en face views of membranes that reveal the pattern of Integral membrane proteins. The E face is the outer lamella of the plasma membrane viewed as if from within the cell, the P face the inner lamella viewed from outside the cell. Fracture planes also often pass along lines of weakness such as the interface between cytoplasm and membrane, so that outer and inner membrane surfaces can be viewed. Further information about the structure can be revealed by freeze etching. Extremely rapid freezing followed by deep etching has allowed the structure of the cytoplasm to be studied without the artefacts that might be introduced by fixation.
(18 Nov 1997)
freeze-drying <technique> Method commonly adopted to produce a dry and stable form of biological material that has not been seriously denatured.
By freezing the specimen, often with liquid nitrogen and then subliming water from the specimen under vacuum, proteins are left in reasonably native form and can usually be rehydrated to an active state. Since the freeze dried material will store without refrigeration for long periods, it is a convenient method for holding back up or reference material or for the distribution of antibiotics, vaccines etc.
Synonym: lyophilization.
(18 Sep 2002)
freeze fracture Method of specimen preparation for the electron microscope in which rapidly frozen tissue is cracked so as to produce a fracture plane through the specimen. The surface of the fracture plane is then shadowed by heavy metal vapour, strengthened by a carbon film and the underlying specimen is digested away, leaving a replica that can be picked up on a grid and examined in the transmission electron microscope. The great advantage of the method is that the fracture plane tends to pass along the centre of lipid bilayers and it is therefore possible to get en face views of membranes that reveal the pattern of Integral membrane proteins. The E face is the outer lamella of the plasma membrane viewed as if from within the cell, the P face the inner lamella viewed from outside the cell. Fracture planes also often pass along lines of weakness such as the interface between cytoplasm and membrane, so that outer and inner membrane surfaces can be viewed. Further information about the structure can be revealed by freeze etching. Extremely rapid freezing followed by deep etching has allowed the structure of the cytoplasm to be studied without the artefacts that might be introduced by fixation.
(18 Nov 1997)
freeze fracturing Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
(12 Dec 1998)
freeze substitution A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low temperature.
(12 Dec 1998)
MeSH(Medical Subject Headings) ¸ÂÃã °Ë»ö (http://www.nlm.nih.gov) °á°ú : 1 ÆäÀÌÁö: 1
  • Freeze Etching - »õâ A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
    Synonyms : Etching, Freeze
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