| fluorescein | <chemical> Fluorophore commonly used in microscopy. Fluorescein di acetate can be used as a vital stain or can be conjugated to proteins (particularly antibodies) using isothiocyanate. Excitation is at 365nm and the emitted light is green yellow (450-490nm). The emission spectrum is pH sensitive and fluorescein can therefore be used to measure pH in intracellular compartments. (18 Nov 1997) |
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| fluorescein-5-isothiocyanate | <chemical> 3',6'-dihydroxy-5-isothiocyanatospiro(isobenzofuran-1(3h),9'-(9h)xanthen)-3-one. Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques. Pharmacological action: fluorescent dyes. Chemical name: Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato- (12 Dec 1998) |
| fluorescein angiography | <procedure> The process of taking X-rays of blood vessels that have been injected with a special dye. The dye allows to person viewing the X-ray to see the blood vessels. (13 Nov 1997) |
| fluorescein-conjugated antibody | Refers to an antibody that has joined with a fluorescein molecule. (09 Oct 1997) |
| fluorescein eye examination | A special test that allows the examiner to detect cornea abrasions or corneal foreign bodies. An orange dye (fluorescein) is placed into the eye and the eye is illuminated with a cobalt blue light (black light). This serves to highlight any irregularities in the corneal surface. (27 Sep 1997) |
| fluorescein instillation test | A test for patency of the lacrimal system; fluorescein instilled in the conjunctival sac can be recovered from the inferior nasal meatus. Synonym: Jones' test. (05 Mar 2000) |
| fluorescein isothiocyanate | <chemical> A fluorescent chemical derived from fluorescein which is used to label proteins. (25 Jun 1999) |
| fluorescein sodium | A dye used for diagnosis of certain ocular diseases, differentiation or delineation of organ parts in surgery, and determination of circulation time. Synonym: resorcinolphthalein sodium, uranin. (05 Mar 2000) |
| fluorescein string test | A string test used to determine location of a bleeding intestinal lesion in which fluorescein is given intravenously to determine gastrointestinal haemorrhage; if the string fluoresces after removal, it has been contaminated by blood that has appeared since injection of the fluorescein; used to determine location of bleeding lesion. (05 Mar 2000) |
| 3-O-methyl-fluorescein phosphatase | <enzyme> Used for assay of na-k-atpase in intact tissues Registry number: EC 3.6.1.- Synonym: 3-o-methylfluorescein phosphatase (26 Jun 1999) |
| azo dye | <chemistry> Dyes that contain the N=N linkage. They are easily prepared from diazo compounds. (18 Nov 1997) |
| radiopaque contrast dye | A radiopaque substance (for example metal) will be highlighted (appear white) on a plain X-ray. The use of iodine containing radiopaque contrast dyes allow enhancement of the anatomy demonstrable with conventional X-ray. (27 Sep 1997) |
| Motulsky dye reduction test | A test for glucose-6-phosphate dehydrogenase deficiency in the blood, using a mixture of brilliant cresyl blue, glucose-6-phosphate, and NADP. (05 Mar 2000) |
| Sabin-Feldman dye test | A method for the detection of anti-toxoplasma antibody in serum, based on the fact that Toxoplasma gondii cells (from peritoneal exudate in mice) are fairly well stained with alkaline methylene blue, whereas organisms in a serum that contains specific antibody have no affinity for the dye; furthermore, normal toxoplasma cells become rounded, and the nucleus and cytoplasm deeply stained, when treated with the methylene blue; on the other hand, when dye is mixed with organisms and antibody, the cells retain their crescent shape and only the shrunken nuclear endosome is stained. (05 Mar 2000) |
| salt dye | <technique> A compound of an acid stain and a basic stain, such as the eosinate of methylene blue, in which the anion and cation each contains a chromophore group. Synonym: salt dye. (05 Mar 2000) |