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eosin-methylene blue agar Agar composed of peptone, lactose, and sucrose and containing eosin and methylene blue, used to distinguish between lactose-fermenting and non-lactose-fermenting Gram-negative bacteria.
Synonym: EMB agar.
(05 Mar 2000)
Mann's methyl blue-eosin stain <technique> A stain useful for anterior pituitary and viral inclusion bodies; a mixture of the two dyes stains alpha cell granules red, beta cell granules dark blue, chromophobes gray to pink, colloid red, erythrocytes orange-red, and collagen fibres blue; this method is also useful for enterochromaffin, goblet, Paneth, and pancreatic islet cells; Negri bodies appear red while their nuclei and central granules are blue.
(05 Mar 2000)
basic fuchsin-methylene blue stain <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink.
(05 Mar 2000)
carbon monoxide-methylene blue oxidoreductase <enzyme> Molybdenum-containing iron-sulfur flavoprotein from pseudomonas carboxydovorans; forms carbon dioxide
Registry number: EC 1.2.3.-
Synonym: cm-mb oxidoreductase, carbon monoxide oxidase
(26 Jun 1999)
methylene blue <chemical> Methylthionine chloride; dark green crystals or crystalline powder having a bronze-like luster, readily reduced to colourless leukomethylene blue, which in turn is readily oxidised to methylene blue. Used as a bacteriologic stain and as an indicator, and administered orally or intravenously in the treatment of congenital methemoglobinaemia and cyanide poisoning.
Pharmacological action: anti-infective agents, urinary, antidotes, dyes.
Chemical name: Phenothiazin-5-ium, 3,7-bis(dimethylamino)-, chloride
(12 Dec 1998)
polychrome methylene blue An alkaline solution of methylene blue which undergoes progressive oxidative demethylation with aging (ripening) to produce a mixture of methylene blue, azures, and methylene violet; boiling with sodium carbonate or other oxidizing agents accomplishes this result quickly, although it is not as highly regarded.
(05 Mar 2000)
new methylene blue A basic thiazin dye, C18H22N3SCl, used for supravital staining of reticulocytes in blood smears.
(05 Mar 2000)
Kuhne's methylene blue Methylene blue in absolute alcohol and phenol solution.
(05 Mar 2000)
Loeffler's methylene blue A stain for diphtheria organisms that contains methylene blue in dilute ethanol plus a slight amount of potassium hydroxide; dye solution gives best results when aged to a polychrome state.
(05 Mar 2000)
alcohol-soluble eosin See: ethyl eosin.
Synonym: alcohol-soluble eosin.
(05 Mar 2000)
haematoxylin and eosin stain <technique> Probably the most generally useful of all staining methods for tissues; nuclei are stained a deep blue-black with haematoxylin, and cytoplasm is stained pink after counterstaining with eosin, usually in water.
(05 Mar 2000)
eosin <protein> A red, fluorescent, bromine-containing, water-insoluble dye usedmainly in toner and cosmetics.
A red or brown potassium or sodium salt of the above dye, it is usedas a biological stain and in pharmaceuticals.
(09 Oct 1997)
eosin B The disodium salt of 4',5'-dibromo-2',7'-dinitrofluorescein.
Synonym: acid red 91, eosin I bluish.
(05 Mar 2000)
eosin I bluish The disodium salt of 4',5'-dibromo-2',7'-dinitrofluorescein.
Synonym: acid red 91, eosin I bluish.
(05 Mar 2000)
eosin y Eosin Ys, the disodium salt of 2',4',5',7'-tetrabromofluorescein.
Synonym: acid red 87, eosin yellowish.
(05 Mar 2000)
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