| HPLC | high-performance liquid chromatography; high-power liquid chromatography; high-pressure liquid chrom... |
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| CCCC | centrifugal countercurrent chromatography |
| FGC | fibrinogen gel chromatography |
| FPLC | fast protein liquid chromatography |
| GC | ganglion cell; gas chromatography; general circulation; general closure; general condition; generali... |
| RP-HPLC | chromatography and reversed-phase high-performance liquid chromatography |
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| AC | Affinity chromatography |
| CGC | Capillary Gas Chromatography |
| CPC | Centrifugal partition chromatography |
| CCC | Counter-current chromatography |
| gradient elution | Elution in column chromatography in which a changing pH or ionic strength is used to separate substances. (05 Mar 2000) |
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| elution | 1. The separation, by washing, of one solid from another. 2. The removal, by means of a suitable solvent, of one material from another that is insoluble in that solvent, as in column chromatography. 3. The removal of antibodies absorbed onto the erythrocyte surface. Synonym: elutriation. Origin: L. E-luo, pp. Lutus, to wash out (05 Mar 2000) |
| elution profile | <chemistry> A graph made to show how much material is being carried out of the column by the eluant in column chromatography over time. The graph will show a number of different peaks, each peak represents a different separated material from the original mixed substance. (09 Oct 1997) |
| elution volume | <chemistry> The amount of eluant which has passed through the column in column chromatography before a particular peak in an elution profile appears, or before a specific substance of interest comes out with the eluant, separated out of the original mixed substance. (09 Oct 1997) |
| absorption chromatography | <investigation> Techniques for separating molecules based on differential absorption and elution. Term for separation methods involving flow of a fluid carrier over a nonmobile absorbing phase. (18 Nov 1997) |
| adsorption chromatography | Chromatography in which separation of substances is achieved by the difference in degree of adsorption of the compounds to a stationary phase. (05 Mar 2000) |
| affinity chromatography | <investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture. (09 Feb 1998) |
| gas chromatography | <technique> A chromatographic technique (a type of column chromatography) in which the stationary phase is solid while the mobile phase is gaseous samples. The gaseous samples are separated based on their different adsorption ability to the solid phase. (09 Oct 1997) |
| gas-liquid chromatography | <technique> A chemistry lab technique, a type of column chromatography, used to separate the components of a mixed substance. The substance is held stationary by an inert solid coated with an inert liquid which is not likely to evaporate (i.e. Is nonvolatile), while a gas (called an eluant) flows past it bringing out the components one at a time. (09 Oct 1997) |
| paper chromatography | <technique> Separation method in which filter paper is used as the support. A type of chromatography in which the stationary phase is a sheet of special-grade filter paper. It is in all other aspects similar to thin-layer chromatography. Not a very sensitive method, but historically important as one of the first methods available for separating natural compounds. (07 Mar 2000) |
| gel exclusion chromatography | <investigation> A lab technique, a type of column chromatography, used to separate the components of a mixture by molecular size and to collect the molecules which are larger than a certain size. It is similar to gel filtration, small molecules are slowed or trapped by the pores in the gel beads filling the column, while large molecules, too large to fit into the pores, slide past the beads and get to the bottom of the column first. at this point, the large molecules are collected. Gel exclusion refers to the maximum size of molecule which will fit into the gel bead pores, and this lab technique is used to collect the molecules in the mixture which are larger than, or excluded from, the pores. (09 Oct 1997) |
| gel filtration chromatography | See: gel filtration. (05 Mar 2000) |
| partition chromatography | The separation of similar substances by repeated divisions between two immiscible liquids, so that the substances, in effect, cross the partition between the liquids in opposite directions; where one of the liquids is bound as a film on filter paper, the process is termed paper partition chromatography or paper chromatography. (05 Mar 2000) |
| reversed phase chromatography | A form of partitionary chromatography in which the stationary phase is more polar than the mobile phase. (05 Mar 2000) |
| chromatography | <investigation> Techniques for separating molecules based on differential absorption and elution. Term for separation methods involving flow of a fluid carrier over a nonmobile absorbing phase. (18 Nov 1997) |
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