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Dieterle's s. a silver impregnation method for staining Legionella and other organisms. Slides are sensitized in uranyl nitrate, treated with gum mastic, incubated in silver nitrate, and developed in a solution of hydroquinone, sodium sulfite, acetone, formaldehyde, pyridine, and gum mastic. Cells stain black on a yellow-tan background.
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