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diazo stain <technique> For argentaffin granules, in enterochromaffin cells, a variety of diazonium salts are used to blacken the cells.
(05 Mar 2000)
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diazo- <chemistry, prefix> A combining form (also used adjectively), meaning pertaining to, or derived from, a series of compounds containing a radical of two nitrogen atoms, united usually to an aromatic radical; as, diazo-benzene, C6H5.N2.OH.
Diazo compounds are in general unstable, but are of great importance in recent organic chemistry. They are obtained by a partial reduction of the salts of certain amido compounds.
<chemistry> Diazo reactions, a series of reactions whereby diazo compounds are employed in substitution. These reactions are of great importance in organic chemistry.
Origin: Pref. Di- + azo-.
(29 Oct 1998)
diazo reaction The reaction of diazotised sulfanilic acid with bilirubin to form azobilirubin, which forms the basis of quantitating the amount of bilirubin in biological fluids.
See: van den Bergh's test.
Synonym: Ehrlich's diazo reaction.
(05 Mar 2000)
diazo reagent Two solutions, one of sodium nitrite, the other of acidified sulfanilic acid, used in bringing about diazotization.
Synonym: Ehrlich's diazo reagent.
(05 Mar 2000)
Ehrlich's diazo reaction The reaction of diazotised sulfanilic acid with bilirubin to form azobilirubin, which forms the basis of quantitating the amount of bilirubin in biological fluids.
See: van den Bergh's test.
Synonym: Ehrlich's diazo reaction.
(05 Mar 2000)
Ehrlich's diazo reagent Two solutions, one of sodium nitrite, the other of acidified sulfanilic acid, used in bringing about diazotization.
Synonym: Ehrlich's diazo reagent.
(05 Mar 2000)
Abbott's stain <technique> Spores are stained blue with alkaline methylene blue; bodies of the bacilli become pink with eosin counterstain.
(05 Mar 2000)
aceto-orcein stain <technique> A stain used for chromosomes in air-dried or squashed cytologic material.
(05 Mar 2000)
acid-fast stain <technique> A staining technique used to determine the cell wall property of a microorganism. After stained with dye such as hot carbolfuschin, an acid-fast organism, (for example Mycobacterium species) will retain the colour in its cell wall after being washed with acid-alcohol.
(13 Nov 1997)
acid stain <technique> A dye in which the anion is the coloured component of the dye molecule, e.g., sodium eosinate (eosin).
(05 Mar 2000)
Ag-AS stain <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin.
Synonym: Ag-AS stain.
(05 Mar 2000)
Albert's stain <technique> A stain for diphtheria bacilli and their metachromatic granules; contains toluidine blue, methyl green, glacial acetic acid, alcohol, and distilled water.
(05 Mar 2000)
Altmann's anilin-acid fuchsin stain <technique> A mixture of picric acid, anilin, and acid fuchsin which stains mitochondria crimson against a yellow background.
(05 Mar 2000)
auramine O fluorescent stain <technique> A rapid and accurate technique for Mycobacterium tuberculosis, using auramine O-phenol and a methylene blue counterstain.
(05 Mar 2000)
basic fuchsin-methylene blue stain <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink.
(05 Mar 2000)
basic stain <technique> A dye in which the cation is the coloured component of the dye molecule that binds to anionic groups of nucleic acids (PO4&equiv;) or acidic mucopolysaccharides (e.g., chondroitin sulfate).
(05 Mar 2000)
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