| denaturation temperature of DNA | That temperature at which, under a given set of conditions, double-stranded DNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., GC content) of the DNA. Synonym: melting temperature of DNA. (05 Mar 2000) |
|---|
| melting temperature of DNA | That temperature at which, under a given set of conditions, double-stranded DNA is changed (50%) to single-stranded DNA; under standard conditions, the base composition of the DNA can be estimated from the denaturation temperature, since the greater the denaturation temperature, the greater the guanine-plus-cytosine content (i.e., GC content) of the DNA. Synonym: melting temperature of DNA. (05 Mar 2000) |
|---|---|
| alkali denaturation test | A test for haemoglobin F (Hb F), based on the fact that haemoglobins, with the exception of Hb F, are denatured by alkali to alkaline haematin; the test is sensitive to 2% or more Hb F. (05 Mar 2000) |
| nucleic acid denaturation | Disorganization of secondary structures of nucleic acids through cleavage of hydrogen bonds and hydrophobic linkages. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible. (12 Dec 1998) |
| denaturation | Reversible or irreversible loss of function in proteins and nucleic acids resulting from loss of higher order secondary, tertiary or quaternary structure) produced by nonphysiological conditions of pH, temperature, salt or organic solvents. (18 Nov 1997) |
| DNA-directed DNA polymerase | <enzyme> DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair. Chemical name: Deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase (DNA-directed) Registry number: EC 2.7.7.7 (12 Dec 1998) |
| absolute temperature | Temperature reckoned in Kelvins from absolute zero. (05 Mar 2000) |
| basal body temperature | <biology> The temperature taken at its lowest point in the day, usually in the morning before getting out of bed. (09 Oct 1997) |
| body temperature changes | Any deviation from normal body temperature of the human body, about 98.6 degrees f. Or 37 degrees c. When taken orally. (12 Dec 1998) |
| maximum temperature | In bacteriology, denoting a temperature above which growth will not take place. (05 Mar 2000) |
| mean temperature | The average atmospheric temperature in any locality for a designated period of time, as a month or a year. (05 Mar 2000) |
| restrictive temperature | <molecular biology> Of a temperature sensitive mutation, a temperature at which the mutated gene product behaves normally and so the cell or organism survives as if wild type. C.f. The restrictive temperature, at which the gene product takes on a mutant phenotype. (18 Nov 1997) |
| melting temperature | The midpoint in the change in optical properties (absorbance, rotation) of a structured polymer (e.g., DNA) with increasing temperature. Synonym: melting temperature. (05 Mar 2000) |
| permissive temperature | <molecular biology> Of a temperature sensitive mutation, a temperature at which the mutated gene product behaves normally and so the cell or organism survives as if wild type. C.f. The restrictive temperature, at which the gene product takes on a mutant phenotype. (18 Nov 1997) |
| room temperature | The ordinary temperature (65 |
| minimum temperature | In bacteriology, denoting a temperature below which growth will not take place. (05 Mar 2000) |