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  • gene cloning
    À¯ÀüÀÚŬ·Î´×, À¯ÀüÀÚº¹Á¦
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    À¯ÀüÀÚŬ·Î´×
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  • DNA cloning
    DNA Ŭ·Î´×, DNA ¼øÁõ½Ä, DNA ¼ø¼öÁõ½Ä
  • gene cloning
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    Ŭ·Î´×, ¼øÁõ½Ä, ¼ø¼öÁõ½Ä
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  • gene cloning
    À¯ÀüÀÚŬ·Î´×
  • positional cloning
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  • cloning
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  • cloning host
    Ŭ·Î´× ¼÷ÁÖ(âÖñ«)
  • cloning vector
    Ŭ·Î´× º¤Å¸
  • cloning vehicle
    Ŭ·Î´× ¿î¹Ýü(ê¡Úæô÷)
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  • cell cloning
    ¼¼Æ÷(á¬øà) Ŭ·Î´×
  • DNA cloning
    "DNA Ŭ·Î´×, (ÔÒ) recombinant DNA technology"
  • gene cloning
    À¯ÀüÀÚ(ë¶îîí­) Ŭ·Î´×
  • lambda cloning vector
    ¶÷´Ù Ŭ·Î´× º¤Å¸
  • molecular cloning
    ºÐÀÚ(ÝÂí­)Ŭ·Î´×
  • plasmid cloning vector
    Çö󽺹̵å Ŭ·Î´× º¤ÅÍ
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CIF cloning inhibitory factor
CLIF cloning inhibitory factor; Crithidia luciliae immunofluorescence
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HTCA Human Tumor Cloning Assay
MCS Multiple cloning sites
%CE cloning efficiency
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  • JrId: 21551
    JournalTitle: Cloning.
    MedAbbr: Cloning
    ISSN: 1520-4553
    ESSN:
    IsoAbbr: Cloning
    NlmId: 100883430
  • JrId: 27067
    JournalTitle: Cloning and stem cells.
    MedAbbr: Cloning Stem Cells
    ISSN: 1536-2302
    ESSN:
    IsoAbbr: Cloning Stem Cells
    NlmId: 101125444
CancerWEB ¿µ¿µ ÀÇÇлçÀü ¸ÂÃã °Ë»ö °á°ú : 6 ÆäÀÌÁö: 1
cloning <molecular biology> The process whereby clones are established asexually, where cells all genetically identical, to a single ancestor.
In recombinant DNA technology, the use of DNA manipulation procedures to produce multiple copies of a single gene or segment of DNA is referred to as cloning DNA.
The term covers various manipulations for isolating and establishing clones. In simple systems single cells may be isolated without precise knowledge of their genotype. In other systems partial or complete selection of chosen genotypes can be manipulated with gene cloning.
In plants the term refers to natural or artificial vegitative propagation.
(12 Mar 1998)
cloning vector <molecular biology> A DNA molecule originating from a virus (plasmid vector), or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication.
Vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. They are also used to insert DNA from one cell type to another.
Examples are plasmids, cosmids, and yeast artificial chromosomes, vectors are often recombinant molecules containing DNA sequences from several sources.
Cloning vectors are usually designed to have convenient restriction sites that can be cut to generate sticky end to which the DNA that is to be cloned can be ligated easily.
(12 Mar 1998)
cloning, cell The process of producing a group of cells (clones), all genetically identical, from a single ancestor.
(12 Dec 1998)
cloning, DNA The use of DNA manipulation procedures to produce multiple copies of a single gene or segment of DNA.
(12 Dec 1998)
cloning, molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
(12 Dec 1998)
cloning, organism The formation of one or more genetically identical organisms derived by vegetative reproduction from a single cell. The source nuclear material can be embryo-derived, foetus-derived, or taken from an adult somatic cell.
(12 Dec 1998)
CancerWEB ¿µ¿µ ÀÇÇлçÀü À¯»ç °Ë»ö °á°ú : 12 ÆäÀÌÁö: 1
gene cloning <molecular biology> The insertion of a DNA sequence into a vector that can then be propagated in a host organism, generating a large number of copies of the sequence.
(18 Nov 1997)
cell cloning The process of producing a group of cells (clones), all genetically identical, from a single ancestral cell.
(12 Dec 1998)
megabase cloning <molecular biology> The cloning of very large DNA fragments.
(29 Oct 1998)
molecular cloning <molecular biology> The biological amplification of a specific DNA sequence through mitotic division of a host cell into which it has been transformed or transfected.
(09 Oct 1997)
complementary DNA cloning <molecular biology, technique> A lab technique where a double-stranded cDNA molecule (or dscDNA) is inserted into a cloning vector (another DNA molecule which will continue to be capable of replication after insertion of foreign material), so that the gene encoded by the cDNA can be expressed (transcribed and used) or so many copies of the gene can be made.
(09 Oct 1997)
multiple cloning site Region of a phage or plasmid vector that has been engineered to contain a series of restriction sites that are usually unique within the entire vector. This makes it particularly easy to insert or excise (subclone) DNA fragments.
(18 Nov 1997)
positional cloning Cloning a gene based simply on knowing its position in the genome without any idea of the function of that gene. Because this is the reverse of how things have been traditionally done, it has also been called reverse genetics.
(12 Dec 1998)
self cloning <molecular biology> Any system in which inappropriate cell types or organisms are eliminated because they possess some character that allows them to die or to remove themselves from the system.
Thus a transfected cell with genetic material including a drug resistance marker will be self cloning in the presence of the drug and nontransfected cells will die.
(18 Nov 1997)
dilution cloning Cloning by diluting the cell suspension to the point at which the probability of there being more than one cell in the inoculum volume is small. Inevitably on quite a few occasions there will not be any cells.
(18 Nov 1997)
directional cloning DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either endof each restriction fragment. This allows the insert to be ligated tothe vector in a specific orientation and prevents the vector fromrecircularising.
(09 Oct 1997)
DNA cloning The use of DNA manipulation procedures to produce multiple copies of a single gene or segment of DNA.
(12 Dec 1998)
expression cloning <molecular biology> A vector that results in the expression of inserted DNA sequences when propagated in a suitable host cell, i.e. The protein coded for by the DNA is synthesised by the host's system.
(06 Aug 1998)
MeSH(Medical Subject Headings) ¸ÂÃã °Ë»ö (http://www.nlm.nih.gov) °á°ú : 2 ÆäÀÌÁö: 1
  • Cloning, Molecular - »õâ The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
    Synonyms :
  • Cloning, Organism - »õâ The formation of one or more genetically identical organisms derived by vegetative reproduction from a single cell. The source nuclear material can be embryo-derived, fetus-derived, or taken from an adult somatic cell.
    Synonyms : Cloning, Clonings, Embryo, Clonings, Human, Clonings, Organism, Embryo Clonings, Human Clonings, Organism Cloning, Organism Clonings
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cloning a general term for the research activity that creates a copy of some biological entity (a gene or organism or cell)
Ãâó: wordnet.princeton.edu/perl/webwn
cloning Cloning is the process of creating an identical copy of an original. A clone in the biological sense, therefore, is a single cell (like bacteria, lymphocytes etc.) or multi-cellular organism that is genetically identical to another living organism. ...
Ãâó: en.wikipedia.org/wiki/Cloning
cloning vector A cloning vector is a small DNA vehicle that carries a foreign DNA fragment. The insertion of the fragment into the cloning vector is done by treating the vehicle and the foreign DNA with the same restriction enzyme, then ligating the fragments together. There are many types of cloning vectors. Plasmids and bacteriophages (such as phage λ) are perhaps most commonly used for this purpose. ...
Ãâó: en.wikipedia.org/wiki/Cloning_vector
cloning vector A small, self-replicating DNA molecule - usually a plasmid or viral DNA chromosome - into which foreign DNA is inserted in the process of cloning genes or other DNA sequences of interest. It can carry inserted DNA and be perpetuated in a host cell. Also called a cloning vehicle, vector, or vehicle.. See vector.
Ãâó: www.fao.org/docrep/003/X3910E/X3910E06.htm
cloning An in vitro procedure in which a particular sequence of DNA (eg, a gene) is reproduced in large amounts by inserting ("splicing") it into a suitable replicon, introducing the resultant recombinant (hybrid) molecule into a cell in which it can replicate, and finally growing the cells in culture. (16)
Ãâó: ppathw3.cals.cornell.edu/glossary/Defs_C.htm
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