| chromosome walking |
A technique that identifies overlapping cloned DNA fragments that form one continuous segment of a chromosome. These fragments can be generated either by random shearing or by partial digestion with a four-base-pair cutter such as Sau3A. A series of colony hybridizations is then carried out, starting with some cloned fragment which has already been identified and which is known to be in the region encompassed by the overlapping clones. ...
출처: www.fao.org/docrep/003/X3910E/X3910E06.htm
|
|---|---|
| chromosome walking |
A technique which produces sets of overlapping DNA clones for studying segments of DNA larger than can be cloned individually. A method for the analysis of large regions of DNA, in which a each end of a large single cloned DNA fragment is used separately to screen recombinant DNA genome library for other clones containing neighbouring sequences.
출처: helios.bto.ed.ac.uk/bto/glossary/c.htm
|
| chromosome walking |
A research technique in which overlapping fragments of a chromosome are checked one at a time in search of a particular gene. It covers smaller regions than chromosome jumping.
출처: www.proteinstrukturfabrik.de/glossary.html
|