| cassette | <molecular biology> A pre existing structure into which an insert can be moved. Fashionably used to refer to certain vectors. See: cassette mechanism. (18 Nov 1997) |
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| cassette mechanism | <molecular biology> Term used for genes such as the a and _ genes that determine mating type in yeast, either one or the other is active. In this gene conversion process, a double stranded nuclease makes a cut at a specific point in the MAT locus, the old gene is replaced with a copy of a silent gene from one or other flanking region and the new copy becomes active. As the process involves replacing one ready made construct with another in an active slot it is called a cassette mechanism. (18 Nov 1997) |
| cassette mutagenesis | The production of mutants within a region (often bounded by unique restriction sites) by the use of synthetic oligonucleotides that fill the gap with mutants designed into the synthetic genetic material. (05 Mar 2000) |