| SB | Southern blotting |
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| WB | Westen blotting |
| WLB | Western Ligand Blotting |
| immunoblotting | blotting |
| blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
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| blotting, northern | Detection of RNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, southern | A method (first developed by e.m. Southern) for detection of DNA that has been electrophoretically separated and immobilised by blotting on nitrocellulose or other type of paper or nylon membrane. (12 Dec 1998) |
| blotting, western | Identification of proteins or peptides that have been electrophoretically separated by blotting and transferred to strips of nitrocellulose paper. The blots are then detected by radiolabelled antibody probes. (12 Dec 1998) |
| Western blotting | <molecular biology> An electroblotting method in which proteins are transferred from a gel to a thin, rigid support (nitrocellulose) and detected by binding of labelled antibody. See: blots. (18 Nov 1997) |
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| Southern blotting | <molecular biology, procedure> General term for the transfer of protein, RNA or DNA molecules from a relatively thick acrylamide or agarose gel or to a paper like membrane (usually nylon or nitrocellulose) by capilliarity or an electric field, preserving the spatial arrangment. Once on the membrane, the molecules are immobilised, typically by baking or by ultra violet irradiation and can then be detected at high sensitivity by hybridisation (in the case of DNA & RNA) or antibody labelling (in the case of protein). RNA blots are called Northern blots, DNA blots, Southern, protein blots, Western. (18 Nov 1997) |
Synonyms : Blot, Far-Western, Blot, West-Western, Far-Western Blot, West-Western Blot, Blot, Far Western, Blot, West Western, Blots, Far-Western, Blots, West-Western, Blotting, Far Western, Blotting, West Western, Blottings, Far-Western, Blottings, West-Western
Synonyms : Blot, Northern, Northern Blot, Blots, Northern, Blottings, Northern, Northern Blots, Northern Blottings
Synonyms : Blot, Southern, Southern Blot
Synonyms : Blot, Southwestern, Southwestern Blot, Blots, Southwestern, Blottings, Southwestern, Southwestern Blots, Southwestern Blottings
Synonyms : Blot, Western, Immunoblot, Western, Western Blot, Western Immunoblot, Blots, Western, Blottings, Western, Immunoblots, Western, Immunoblottings, Western, Western Blots, Western Blottings, Western Immunoblots, Western Immunoblottings
| blotting |
(blot
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| blotting |
Following electrophoresis: the transfer of nucleic acids and/or proteins from a gel strip to a specialized, chemically reactive matrix on which the nucleic acids, etc. may become covalently bound in a pattern similar to that present in the original gel. (16)
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| blotting method |
A technique for analyzing a tiny portion of the primary structure of genomic material (DNA or RNA).
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| blotting | absorbent paper used to dry ink |
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