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batch culture A closed system culture of microorganisms with specific nutrient types, temperature, pressure, aeration, and other environmental conditions, where only a few generations are allowed to grow before all nutrients are used up.
Compare: continuous culture.
(09 Oct 1997)
batch processing <technique> Growth in a closed system with a specific amount of nutrient medium. In bioprocessing, defined amounts of nutrient material and living matter are placed in a bioreactor and removed when the process is completed.
(14 Nov 1997)
gradient elution Elution in column chromatography in which a changing pH or ionic strength is used to separate substances.
(05 Mar 2000)
elution 1. The separation, by washing, of one solid from another.
2. The removal, by means of a suitable solvent, of one material from another that is insoluble in that solvent, as in column chromatography.
3. The removal of antibodies absorbed onto the erythrocyte surface.
Synonym: elutriation.
Origin: L. E-luo, pp. Lutus, to wash out
(05 Mar 2000)
elution profile <chemistry> A graph made to show how much material is being carried out of the column by the eluant in column chromatography over time. The graph will show a number of different peaks, each peak represents a different separated material from the original mixed substance.
(09 Oct 1997)
elution volume <chemistry> The amount of eluant which has passed through the column in column chromatography before a particular peak in an elution profile appears, or before a specific substance of interest comes out with the eluant, separated out of the original mixed substance.
(09 Oct 1997)
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