| ACIF | anticomplement immunofluorescence |
|---|---|
| AC | abdominal circumference; abdominal compression; absorption coefficient; abuse case; acetate; acetylc... |
| ACA | abnormal coronary artery; acrodermatitis chronica atrophicans; acute cerebellar ataxia; adenocarcino... |
| CLIF | cloning inhibitory factor; Crithidia luciliae immunofluorescence |
| DIF | diffuse interstitial fibrosis; direct immunofluorescence; dose increase factor |
| ACIF | Anticomplement immunofluorescence |
|---|---|
| DIF | Direct Immunofluorescence |
| DFA | Direct immunofluorescence assay |
| IF | Immunofluorescence |
| IFA | Immunofluorescence |
| anticomplement immunofluorescence | A technique used to make certain indirect fluorescent antibody techniques more specific and sensitive. Here the fluorescent dye is conjugated to antibody directed at complement and then added to a complement-fixing complex of antigen and patient antibody. (05 Mar 2000) |
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| anticomplement | A substance that combines with a complement and so neutralises its action by preventing its union with the antibody. Synonym: antialexin. (05 Mar 2000) |
|---|---|
| avidin-biotin immunofluorescence | Holds promise for more sensitive and specific amplification of indirect fluorescent antibody procedures. Antibody to the patient's specific antibodies is labelled with biotin, a compound capable of specifically binding avidin in high concentrations. Fluorescent labelled avidin is then added and fluorescent microscopy is used to detect the presence of the complexes. (05 Mar 2000) |
| micro-immunofluorescence | Several different substrates are arranged in specific locations on a single microscope slide well allowing a rapid, simultaneous indirect fluorescent antibody on each substrate. (05 Mar 2000) |
| immunofluorescence | <technique> A test or technique in which one or other component of an immunological reaction is made fluorescent by coupling with a fluorochrome such as fluorescein, phycoerythrin or rhodamine so that the occurrence of the reaction can be detected as a fluorescing antigen-antibody complex. Used in microscopy to localise small amounts of antigen or specific antibody. (18 Nov 1997) |
| immunofluorescence method | Any method in which a fluorescent-labelled antibody is used to detect the presence or determine the location of the corresponding antigen. (05 Mar 2000) |
| indirect immunofluorescence | <procedure> A method of immunofluorescence staining in which the first antibody, that is directed against the antigen to be localised, is used unlabelled and the location of the first antibody is then detected by use of a fluorescently labelled antiIgG (against IgGs of the species in which the first antibody was raised). The advantage is that there is some amplification and a well characterised goat antirabbit IgG antibody can, for example: be used against a scarce specific antibody raised in rabbits. The same technique can be used for ultrastructural localisation of the first antibody by substituting peroxidase or gold labelled second antibody. (18 Nov 1997) |
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