| affinity chromatography |
that based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand. Any of these substances, covalently linked to an insoluble support or immobilized in a gel, may serve as the sorbent allowing the interacting substance to be isolated from relatively impure samples; often a 1000-fold purification can be achieved in one step.
출처: www.mercksource.com/pp/us/cns/cns_hl_dorlands.jspz...
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| affinity chromatography |
This was performed at room temperature. A column (volume 600 mL, diameter 10 cm) of 10 carboxydecyl Sepharose was equilibrated with 0.01 m Tris/HCl buffer, pH 7.5. The enzyme preparation ( 1200 mL, clarified by centrifugation if necessary) was loaded at a flow of about l L
출처: dx.doi.org/10.1046/j.1432-1327.1999.00005.x
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