| IWB | indeterminate [HIV-1] Western blot; index of well being |
|---|---|
| WB | waist belt; washable base; washed bladder; water bottle; Wechsler-Bellevue [Scale]; weight-bearing; ... |
| WE | wax ester; Wernicke encephalopathy; western encephalitis; western encephalomyelitis; wound of entry |
| RIBA | Recombinant Immuno-Blot Assay |
| BLOT | British Library of Tape |
| WB | Western blot |
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| WB | Western Blot analysis |
| WLB | Western Ligand Blot |
| immunoblot | immunosorbent assay and Western blot |
| EITB | Enzyme Linked Immunoelectro Transfer Blot |
| western blot | <technique> A technique similar to Southern blotting, though it is used for proteins. (09 Oct 1997) |
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| Western blot analysis | A procedure in which proteins separated by electrophoresis in polyacrylamide gels are transferred (blotted) onto nitrocellulose or nylon membranes and identified by specific complexing with antibodies that are either pre-or post-tagged with a labelled secondary protein. See: immunoblot. Synonym: Western blot, Western blotting. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| Western blotting | <molecular biology> An electroblotting method in which proteins are transferred from a gel to a thin, rigid support (nitrocellulose) and detected by binding of labelled antibody. See: blots. (18 Nov 1997) |
| blot, western | A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
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| blot | 1. To spot, stain, or bespatter, as with ink. "The brief was writ and blotted all with gore." (Gascoigne) 2. To impair; to damage; to mar; to soil. "It blots thy beauty, as frosts do bite the meads." (Shak) 3. To stain with infamy; to disgrace. "Blot not thy innocence with guiltless blood." (Rowe) 4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. "One act like this blots out a thousand crimes." (Dryden) 5. To obscure; to eclipse; to shadow. "He sung how earth blots the moon's gilded wane." (Cowley) 6. To dry, as writing, with blotting paper. Synonym: To obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch. Origin: Cf. Dan. Plette. See Blot. Source: Websters Dictionary (01 Mar 1998) |
| blot, northern | A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). (12 Dec 1998) |
| blot, southern | A common test for checking for a match between DNA molecules. DNA fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X Files a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome). The Southern blot is named after its inventor, the British biologist M.E. Southern. There is also a Northern blot and a Western blot. (12 Dec 1998) |
| southern blot | A technique used for searching for a specific DNA fragment. The process is as follows: 1. separate DNA fragments by gel electrophoresis 2. Change pH of gel to basic, thus allowing disruption of H-bonds 3. blot gel with nitrocellulose paper 4. Heat paper so as to fix DNA fragments 5. probe with labelled messenger RNA or cDNA 6. wash Complementary mRNA/cDNA fragments will have hybridised. (09 Oct 1997) |
| Southern blot analysis | A procedure to separate and identify DNA sequences; DNA fragments are separated by electrophoresis on an agarose gel, transferred (blotted) onto a nitrocellulose or nylon membrane, and hybridised with complementary (labelled) nucleic acid probes. (05 Mar 2000) |
| Northern blot | <molecular biology, procedure> An electroblotting method in which RNA is transferred to a filter and detected by hybridisation to (32)P labelled RNA or DNA. (18 Nov 1997) |
| Northern blot analysis | A procedure similar to the Southern blot analysis, used mostly to separate and identify RNA fragments; typically via transferring RNA fragments from an agarose gel to a nitrocellulose filter followed by detection with a suitable probe. Origin: coined to distinguish it from eponymic Southern blot a. (05 Mar 2000) |
| dot blot | <molecular biology, procedure> Method for detecting a specific protein or message. A spot of solution is dotted onto nitrocellulose paper, a specific antibody or probe is allowed to bind and the presence of bound antibody/probe then shown by using a peroxidase coupled second antibody, as in Western blotting or by other visualisation methods. (18 Nov 1997) |
| ink blot tests | Projective tests utilizing ink blots to which a subject responds. They are used in personality diagnosis. (12 Dec 1998) |
| zoo blot analysis | A procedure using Southern blot analysis to test the ability of a nucleic acid probe from one species to hybridise with the DNA fragment of another species. (05 Mar 2000) |
| africa, western | The geographical area of africa comprising benin, burkina faso, cote d'ivoire, gambia, ghana, guinea, guinea-bissau, liberia, mali, mauritania, niger, nigeria, senegal, sierra leone, and togo. (12 Dec 1998) |
| asia, western | The geographical designation for the countries of the middle east and the countries bangladesh, bhutan, india, nepal, pakistan, and sri lanka. (12 Dec 1998) |
| blotting, western | Identification of proteins or peptides that have been electrophoretically separated by blotting and transferred to strips of nitrocellulose paper. The blots are then detected by radiolabelled antibody probes. (12 Dec 1998) |
| western | 1. Of or pertaining to the west; situated in the west, or in the region nearly in the direction of west; being in that quarter where the sun sets; as, the western shore of France; the western ocean. "Far o'er the glowing western main." (Keble) 2. Moving toward the west; as, a ship makes a western course; coming from the west; as, a western breeze. Western Church. See Latin Church, under Latin. Western empire, the western portion of the Roman empire, as divided, by the will of Theodosius the Great, between his sons Honorius and Arcadius, a. D. 395. Source: Websters Dictionary (01 Mar 1998) |
| Western blot test |
A technique for analyzing protein antigens. Initially, the antigens are separated by electrophoresis on a gel and transferred to a solid membrane by blotting. The membrane is incubated with antibodies, and then the bound antibo
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| Western blotting method |
A technique for analyzing protein antigens.
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| Western blot a. |
see under technique.
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| Western blot h. |
see under technique.
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| Western blot t. |
a technique for analyzing and identifying protein antigens: the proteins are separated by electrophoresis in polyacrylamide gel, then transferred (“blotted”) onto a nitrocellulose membrane or treated paper, where they bind in the same pattern as they formed in the gel. The antigen is overlaid first with antibody, then with anti-immunoglobulin or protein A labeled with a radioisotope, fluorescent dye, or enzyme.
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