| Perls' Prussian blue stain | <technique> A stain for ferric iron as in haemosiderins, using potassium ferrocyanide in acetic acid or dilute hydrochloric acid followed by a red counterstain such as safranin O or neutral red; various haemosiderins and most mineral irons give a blue-green reaction, while nuclei stain red. (05 Mar 2000) |
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| Prussian blue stain | <technique> A stain employing acid potassium ferrocyanide to demonstrate iron, as in siderocytes. (05 Mar 2000) |
| Prussian blue | Fe4(Fe(CN)6)3; ferric ferrocyanide;a dye used to colour injection masses for blood vessels and lymphatics, and in staining of siderocytes. Synonym: Prussian blue. (05 Mar 2000) |
| prussian blue reaction | The reaction of potassium ferrocyanide with ferric iron to yield a dark blue precipitate at the sites of the ferric iron. Used to determine ferric iron in tissues, particularly in the diagnosis of disorders of iron metabolism. (12 Dec 1998) |
| Perls | Max, German pathologist, 1843-1881. See: Perls' Prussian blue stain, Perls' test. (05 Mar 2000) |
| Perls' test | A test for haemosiderin, utilizing Perls' Prussian blue stain. (05 Mar 2000) |
| prussian | Of or pertaining to Prussia. A native or inhabitant of Prussia. <chemistry> Prussian blue Same as Berlin green, under Berlin. Origin: From Prussia, the country: cf. F. Prussien. Source: Websters Dictionary (01 Mar 1998) |
| basic fuchsin-methylene blue stain | <technique> A stain for intact epoxy sections; semi-thick sections of plastic-embedded tissues have nuclei stained purple; collagen, elastic lamina, and connective tissue are stained blue; mitochondria, myelin, and lipid droplets are stained red; cytoplasm, smooth muscle cells, axoplasm, and chrondroblasts are stained pink. (05 Mar 2000) |
| Borrel's blue stain | <technique> A stain for demonstrating spirochetes, treponemes, and Borrelia organisms, using silver oxide (prepared by means of mixing solutions of silver nitrate and sodium bicarbonate) and methylene blue. (05 Mar 2000) |
| Mallory's aniline blue stain | <technique> A method especially suitable for studying connective tissue; sections are stained in acid fuchsin, aniline blue-orange G solution, and phosphotungstic acid; fibrils of collagen are blue, fibroglia, neuroglia, and muscle fibres are red, and fibrils of elastin are pink or yellow. Synonym: Mallory's aniline blue stain, Mallory's triple stain. (05 Mar 2000) |
| Mann's methyl blue-eosin stain | <technique> A stain useful for anterior pituitary and viral inclusion bodies; a mixture of the two dyes stains alpha cell granules red, beta cell granules dark blue, chromophobes gray to pink, colloid red, erythrocytes orange-red, and collagen fibres blue; this method is also useful for enterochromaffin, goblet, Paneth, and pancreatic islet cells; Negri bodies appear red while their nuclei and central granules are blue. (05 Mar 2000) |
| Kluver-Barrera Luxol fast blue stain | <technique> In combination with cresyl violet, a stain useful for demonstrating myelin and Nissl substance. (05 Mar 2000) |
| Lillie's sulfuric acid Nile blue stain | <technique> A technique for showing fatty acids when present in high concentrations. (05 Mar 2000) |
| alcian blue | <chemical> Water soluble copper phthalocyanin stain used to demonstrate acid mucopolysaccharides. By varying the ionic strength some differentiation of various types is possible. (18 Nov 1997) |
| alkaline toluidine blue O | Toluidine blue O in borax solution, used with heat on semithick sections of epoxy embedded tissues. (05 Mar 2000) |