| base sequence analysis | <molecular biology> A method, sometimes automated, for determining the base sequence. (09 Oct 1997) |
|---|---|
| sequence analysis | A multistage process that includes the determination of a sequence (protein, carbohydrate, etc.), its fragmentation and analysis, and the interpretation of the resulting sequence information. This information is useful in that it: 1) reveals the similarities of homologous genes, thereby providing insight into the possible regulation and functions of these genes; and 2) leads to a better understanding of disease states related to genetic variation. New sequencing methodologies, fully automated instrumentation, and improvements in sequencing-related computational resources contribute to the potential for genome-size sequencing projects. (12 Dec 1998) |
| sequence analysis, DNA | A multistage process that includes DNA cloning, physical mapping, subcloning, sequencing, and information analysis. New technological advances have led to the automation of certain steps in this process and contribute to the potential for large-scale DNA sequencing efforts. (12 Dec 1998) |
| sequence analysis, RNA | A multistage process that includes RNA cloning, physical mapping, subcloning, sequencing, and information analysis. (12 Dec 1998) |
| DNA sequence analysis | <molecular biology> Determination of the nucleotide sequence of a length of DNA. Typically, this is performed by cloning the DNA of interest, so that enough can be prepared to allow the sequence to be determined, usually by the Sanger dideoxy chain temination or Maxam Gilbert chain degradation techniques. The resulting reactions are then run on a large sequencing gel, capable of resolving single nucleotide differences in chain length. Recently, PCR based methods have obviated the need to clone the DNA under some conditions and automated DNA sequencing has become widely available. (18 Nov 1997) |
| deoxynucleoside diphosphate-oligonucleotide deoxynucleotidyl transferase | <chemical> Activated by mg++ Synonym: dndp-deoxynucleotidyl transferase, ddodt (26 Jun 1999) |
| oligonucleotide | <biochemistry> Linear sequence of up to 20 nucleotides joined by phosphodiester bonds. Above this length the term polynucleotide begins to be used. (18 Nov 1997) |
| oligonucleotide probes | Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridises with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32p and 125i and the chemical label biotin. (12 Dec 1998) |
| alu sequence | Any of a family of short (300 basepairs long) repeated sequences that occur throughout the human genome. (09 Oct 1997) |
| amino acid sequence | The sequence of amino acids as arrayed in chains, sheets, etc., within the protein molecule. This is referred to as the primary structure of proteins. It is of fundamental importance in determining protein conformation. (12 Dec 1998) |
| autonomously replicating sequence | <molecular biology> This is a chromosomal sequence that allows plasmids to replicate on their own in yeast. (02 Jan 1998) |
| base sequence | <molecular biology> The order of nucleotide bases in a DNA molecule. (09 Oct 1997) |
| canonical sequence | Of a series of related DNA, RNA or protein sequences, the sequence that reflects the most common choice of base or amino acid at each position. Areas of particularly good agreement often represent conserved functional domains. The generation of consensus sequences has been subjected to intensive mathematical analysis. (18 Nov 1997) |
| carbohydrate sequence | The sequence of carbohydrates within polysaccharides, glycoproteins, and glycolipids. (12 Dec 1998) |
| palindromic sequence | <molecular biology> Nucleic acid sequence that is identical to its complementary strand when each is read in the correct direction (e.g. TGGCCA). Palindromic sequences are often the recognition sites for restriction enzymes. Degenerate palindromes with internal mismatching can lead to loops or hairpins being formed (as in tRNA). (18 Nov 1997) |