| Lineweaver-Burk equation |
a rearrangement of the Michaelis-Menten equation of enzyme kinetics to give
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| Lineweaver-Burk e. |
a rearrangement of the Michaelis-Menten equation of enzyme kinetics to give 1v = KMVmax[S] + 1Vmax , where v is the reaction velocity, [S] is the substrate concentration, Vmax is the maximum velocity, and KM is the Michaelis constant. If an enzyme reaction follows Michaelis-Menten kinetics, then a plot of 1/v against 1/[S] results in a straight line with defined slope and intercepts; see Lineweaver-Burk plot, under plot.
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| Lineweaver-Burk equation, plot |
see under equation.
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| Lineweaver-Burk p. |
the double reciprocal plot of an enzyme-catalyzed reaction, obtained from the Lineweaver-Burk equation by graphing 1/v as a function of 1/[S]. If the reaction obeys Michaelis-Menten kinetics a straight line is obtained with x-intercept of -1/KM, y-intercept of 1/Vmax, and slope of KM/Vmax. Although unreliable for large values of 1/v, the plot is widely used. It is also sometimes used in the classification of enzyme inhibitors, which all cause distinctive alterations in the graph. Click here to view image■Lineweaver-Burk plot.
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