| 영문 | Gram stain | 한글 | 그람염색 |
|---|---|---|---|
| 설명 | 미생물을 염색하는 방법 중의 하나. 1884년 덴마크의 의사 H.C.J. 그람(1853~ 1938)이 고안한 특수 염색법이다. 이 염색법을 사용하면 미생물은 양성균과 음성균의 2무리로 크게 나뉜다. 염색법은 균을 유리면에 바르고 가열 고정한다. 젠시안바이오렛, 크리스탈 바이오렛, 메틸바이오렛 등 파라로즈 아닐린계 색소의 어느 하나를 사용하여 우선 염색하고 이어서 루골액을 작용시킨 뒤, 에탄올 또는 아세톤으로 탈색한다. 이때 탈색되지 않고 보라색을 타나내는 것을 그람양성균이라 부르며, 포도알균-폐렴알균-사슬알균 등이 대표적인 예이다. 탈색되면 그람음성균이라 부르며, 대장균-녹농균 등이 있다. 최후에 사프라닌, 푹신 등 적색계의 색소액으로 후염색을 한다. 미생물의 감별에 중요한 염색법이며 염색성의 차이는 영양요구성, 화학요법제(항생물질 등)에 대한 감수성의 차이와도 관련되는 점이 많으므로 사용약제의 선택시에도 중요한 역할을 한다. 그람양성균과 음성균의 차이는 세균벽의 구조에 있는데, 양성균은 세포막의 바깥쪽 펩티드글리칸이라는 당단백질층의 두께가 20~80nm나 되고, 그램음성균의 펩티드글리칸 층은 2~3nm로 얇고, 다시 바깥쪽에 외막이라 부르는 층이 있다. |
||
| GMS | General Medical Service; geriatric mental state; Gilbert-Meulengracht syndrome; Gomori methenamine s... |
|---|---|
| WS | Waardenburg syndrome; ward secretary; Warkany syndrome; Warthin-Starry [stain]; water soluble; water... |
| AFB Stain | Acid Fast Bacilli Stain |
| HE stain | Hematoxylin-Eosin stain |
| Ag | antigen; silver [Lat. argentum] |
| GMS | Gomori Methenamine-Silver |
|---|---|
| PWS | Port Wine Stain |
| IGSS | Immuno-Gold-Silver staining |
| PA-TCH-SP | Periodate-thiocarbohydrazide-silver proteinate |
| PA-TCH-SP | Periodic acid-thiocarbohydrazide silver proteinate |
| Gomori's silver impregnation stain | <technique> A reliable method for reticulin, as an aid in the diagnosis of neoplasm and early cirrhosis of the liver; the staining solution employs silver nitrate, potassium hydroxide, and ammonia water carefully prepared to avoid having silver precipitate. (05 Mar 2000) |
|---|---|
| Gomori-Jones periodic acid-methenamine-silver stain | <technique> A staining method using methenamine silver, periodic acid, gold chloride, haematoxylin, and eosin to delineate basement membrane, reticulin, collagen, and nuclei; used in renal histopathology. See: Rambourg's periodic acid-chromic methenamine-silver stain. (05 Mar 2000) |
| Gomori's methenamine-silver stain | <technique> Techniques for 1) argentaffin cells: a method using a methenamine-silver solution in combination with gold chloride, sodium thiosulphate, and safranin O; argentaffin granules appear brown-black against a green background; 2) urates: warm sections are treated directly with a hot methenamine-silver solution to produce a blackening of urates; 3) fungi: see Grocott-Gomori methenamine-silver stain; 4) melanin, which reduces silver nitrate. (05 Mar 2000) |
| Grocott-Gomori methenamine-silver stain | <technique> A modification of Gomori's methenamine-silver stain for fungi in which sections are pretreated with chromic acid before addition of the methenamine-silver solution and then counterstained with light green to demonstrate black-brown fungi against a pale green background. (05 Mar 2000) |
| silver impregnation | Silver complexes employed to demonstrate reticulin in normal and diseased tissues, as well as neuroglia, neurofibrillae, argentaffin cells, and Golgi apparatus. (05 Mar 2000) |
| silver-ammoniacal silver stain | <technique> A stain for the acid protein component of nucleolar regions which are active or which were transcriptionally active in the preceding interphase; uses silver nitrate, ammoniacal silver, and formalin. Synonym: Ag-AS stain. (05 Mar 2000) |
| Foot's reticulin impregnation stain | <technique> A silver stain in which reticulin stains black and collagen stains golden brown; sections are floated on the surface of solutions to avoid contamination with silver debris. (05 Mar 2000) |
| Gomori's aldehyde fuchsin stain | <technique> A stain used to demonstrate beta cells of the pancreas, storage form of thyrotrophic hormone in beta cells of the anterior pituitary, hypophyseal neurosecretory substance, mast cells, granules, elastic fibres, sulfated mucins, and gastric chief cells. (05 Mar 2000) |
| Gomori's chrome alum haematoxylin-phloxine stain | <technique> A technique used to demonstrate cytoplasmic granules, after Bouin's or formalin-Zenker fixatives, using oxidised haematoxylin plus phloxine; in the pancreas, beta cells are blue, alpha and delta cells are red, and zymogen granules are red to unstained; in the pituitary, alpha cells are pink, beta cells and chromophobes are gray-blue, and nuclei are purple to blue. (05 Mar 2000) |
| Gomori's non-specific acid phosphatase stain | <technique> A method in which formalin-fixed frozen sections are incubated in a substrate containing sodium beta-glycerophosphate and lead nitrate at pH 5.0; the insoluble lead phosphate produced is treated with ammonium sulfide to give a black lead sulfide. (05 Mar 2000) |
| Gomori's non-specific alkaline phosphatase stain | <technique> A calcium-cobalt sulfide method using frozen sections or cold acetone-or formalin-fixed paraffin sections, plus sodium beta-glycerophosphate as a substrate at pH 9.0 to 9.5 with Mg++ as activator; calcium ions precipitate the liberated phosphate, cobalt salt replaces the calcium phosphate, and ammonium sulfide converts the product to a black cobalt sulfide. (05 Mar 2000) |
| Gomori's one-step trichrome stain | <technique> A connective tissue stain that uses haematoxylin and a dye mixture containing chromotrope 2R and light green or aniline blue; muscle fibres appear red, collagen is green (or blue if aniline blue is used), and nuclei are blue to black. (05 Mar 2000) |
| impregnation | 1. <gynaecology> The act of fecundation or of rendering pregnant. 2. The process or act of saturation, a saturated condition. Origin: L. Impregnatio (18 Nov 1997) |
| Rambourg's periodic acid-chromic methenamine-silver stain | <technique> A stain for glycoproteins, used with an electron microscope, adapted from the Gomori-Jones periodic acid-methenamine-silver stain; it produces silver deposits in mature saccules of the Golgi apparatus, lysosomal vesicles, cell coat, and basement membranes. (05 Mar 2000) |
| Masson-Fontana ammoniacal silver stain | <technique> A stain used to demonstrate melanin and argentaffin granules. Synonym: Fontana-Masson silver stain. (05 Mar 2000) |
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|
제품명 |
판매사 |
보험코드 | 성분/함량 | 구분/보험급여 |
|---|