| Kasten's fluorescent Feulgen stain | <technique> A fluorescent modification of the Feulgen stain, utilizing any one of a variety of fluorescent basic dyes to which SO2 is added; the brilliant fluorescence makes this method unusually sensitive and adaptable to cytofluorometric quantification of DNA. (05 Mar 2000) |
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| Feulgen reaction | <procedure> A specific staining procedure for DNA: mild acid hydrolysis makes the aldehyde group of deoxyribose available to react with Schiff's reagent to give a purple colour. (18 Nov 1997) |
| Feulgen reagent | <chemical> A staining substance which specifically stains DNA. Robert Feulgen, in 1914, used this stain to find out that DNA is the hereditary material in cells. (09 Oct 1997) |
| Feulgen, Robert | <person> German nucleic acid biochemist and cytochemist, 1884-1955. First to detect DNA in cells by a specific cytochemical test. (05 Mar 2000) |
| Feulgen stain | <technique> A selective cytochemical reaction for DNA in which sections or cells are first hydrolyzed with hydrochloric acid to produce apurinic acid and then are stained with Schiff's reagent to produce magenta-stained nuclei; generally the concentration of DNA in nucleoli and mitochondria is too low to permit detection by this stain See: Kasten's fluorescent Feulgen stain. (05 Mar 2000) |
| Abbott's method | A method of treatment of scoliosis by use of a series of plaster jackets applied after partial correction of the curvature by external force. (05 Mar 2000) |
| Abell-Kendall method | A standard method for estimation of total serum cholesterol involving saponification of cholesterol ester by hydroxide, extraction with petroleum ether, and colour development with acetic anhydride-sulfuric acid; the method avoids interference by bilirubin, protein, and haemoglobin. (05 Mar 2000) |
| activated sludge method | A method of sewage disposal in which the sewage is treated with 15% bacterially active, liquid sludge, which is produced by repeated vigorous aeration of fresh sewage to form floccules or sediment; when this flocculation process is complete, the resulting activated sludge contains large numbers of bacteria, together with yeasts, molds, and protozoa, which actively effect the oxidation of organic compounds; this mixture is piped to a sedimentation tank, the effluent from which is completely treated sewage. (05 Mar 2000) |
| Altmann-Gersh method | The method of rapidly freezing a tissue and dehydrating it in a vacuum. (05 Mar 2000) |
| Anel's method | Ligation of an artery immediately above (on the proximal side of) an aneurysm. (05 Mar 2000) |
| Antyllus' method | Ligation of the artery above and below an aneurysm, followed by incision into and emptying of the sac. (05 Mar 2000) |
| aristotelian method | A method of study that stresses the relation between a general category and a particular object. (05 Mar 2000) |
| Ashby method | A differential agglutination method for estimating erythrocyte life span; compatible blood possessing a group factor that the recipient lacks is transferred to the recipient; after the transfusion, sera with potent agglutinins for the recipient's red cells are added to samples of the recipient's blood, and the unagglutinated red cells are counted; using this technique the red cell life span in normal persons is found to be 110 to 120 days. (05 Mar 2000) |
| auxanographic method | A method for the study of bacterial enzymes in which agar is mixed with the material (e.g., starch or milk) which is to serve as an indicator of the enzyme action and is inoculated and plated; if the bacteria produce enzymes digesting the admixed material, there will be a zone of clearing in the medium about each colony. Synonym: diffusion method. (05 Mar 2000) |
| Barraquer's method | Zonulysis Dissolution of the zonula ciliaris by enzymes (alpha-chymotrypsin) to facilitate surgical removal of a cataract. Synonym: Barraquer's method. Origin: zonule + G. Lysis, dissolution (05 Mar 2000) |