| Feldman, Harry Alfred | <person> U.S. Epidemiologist, *1914. See: Sabin-Feldman dye test. (05 Mar 2000) |
|---|
| Sabin-Feldman dye test | A method for the detection of anti-toxoplasma antibody in serum, based on the fact that Toxoplasma gondii cells (from peritoneal exudate in mice) are fairly well stained with alkaline methylene blue, whereas organisms in a serum that contains specific antibody have no affinity for the dye; furthermore, normal toxoplasma cells become rounded, and the nucleus and cytoplasm deeply stained, when treated with the methylene blue; on the other hand, when dye is mixed with organisms and antibody, the cells retain their crescent shape and only the shrunken nuclear endosome is stained. (05 Mar 2000) |
|---|