| Edman method | C6H5-N==C==S, a reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids. Synonym: Edman's reagent. Acronym: PITC (05 Mar 2000) |
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| edman degradation | A lab technique used to find out the order of amino acids in a polypeptide (chain of amino acids). It involves using the Edman reagent, phenyl isothiocyanate (PITC), to react one by one with each amino acid, in order. The technique is used in machines which automatically sequence (determine the order of subunits) polypeptides. (09 Oct 1997) |
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| Edman, Pehr | <person> Australian scientist, 1916-1977. See: Edman method, Edman's reagent. (05 Mar 2000) |
| Edman reagent | Phenyl isothiocyanate. The classic method for sequence determination of peptides using sequential cleavage of the N terminal residue after reaction with Edman reagent. The N terminal amino acid is removed as a phenylthiohydantoin derivative. (18 Nov 1997) |
| Edman's reagent | C6H5-N==C==S, a reagent that condenses with the free N-terminal amino group of a peptide chain to form a phenylthiohydantoin in the Edman method of identifying N-terminal amino acids. Synonym: Edman's reagent. Acronym: PITC (05 Mar 2000) |
| Abbott's method | A method of treatment of scoliosis by use of a series of plaster jackets applied after partial correction of the curvature by external force. (05 Mar 2000) |
| Abell-Kendall method | A standard method for estimation of total serum cholesterol involving saponification of cholesterol ester by hydroxide, extraction with petroleum ether, and colour development with acetic anhydride-sulfuric acid; the method avoids interference by bilirubin, protein, and haemoglobin. (05 Mar 2000) |
| activated sludge method | A method of sewage disposal in which the sewage is treated with 15% bacterially active, liquid sludge, which is produced by repeated vigorous aeration of fresh sewage to form floccules or sediment; when this flocculation process is complete, the resulting activated sludge contains large numbers of bacteria, together with yeasts, molds, and protozoa, which actively effect the oxidation of organic compounds; this mixture is piped to a sedimentation tank, the effluent from which is completely treated sewage. (05 Mar 2000) |
| Altmann-Gersh method | The method of rapidly freezing a tissue and dehydrating it in a vacuum. (05 Mar 2000) |
| Anel's method | Ligation of an artery immediately above (on the proximal side of) an aneurysm. (05 Mar 2000) |
| Antyllus' method | Ligation of the artery above and below an aneurysm, followed by incision into and emptying of the sac. (05 Mar 2000) |
| aristotelian method | A method of study that stresses the relation between a general category and a particular object. (05 Mar 2000) |
| Ashby method | A differential agglutination method for estimating erythrocyte life span; compatible blood possessing a group factor that the recipient lacks is transferred to the recipient; after the transfusion, sera with potent agglutinins for the recipient's red cells are added to samples of the recipient's blood, and the unagglutinated red cells are counted; using this technique the red cell life span in normal persons is found to be 110 to 120 days. (05 Mar 2000) |
| auxanographic method | A method for the study of bacterial enzymes in which agar is mixed with the material (e.g., starch or milk) which is to serve as an indicator of the enzyme action and is inoculated and plated; if the bacteria produce enzymes digesting the admixed material, there will be a zone of clearing in the medium about each colony. Synonym: diffusion method. (05 Mar 2000) |
| Barraquer's method | Zonulysis Dissolution of the zonula ciliaris by enzymes (alpha-chymotrypsin) to facilitate surgical removal of a cataract. Synonym: Barraquer's method. Origin: zonule + G. Lysis, dissolution (05 Mar 2000) |
| Beck's method | A permanent opening into the stomach made from its greater curvature. (05 Mar 2000) |