영어로 된 문헌에서 PCR subtraction library cloning이라는 기술이 언급되고 이러한 클로닝에 의해 얻어지는 서열을 subtracted cDNA sequences라고 표현하는데, 여기서 subtraction이란 어떤 의미를 갖는 건가요? 새로운 PCR 기법인가요? 아시는 분 답변 부탁드립니다.
Substraction library:
A cDNA library that only contains cDNAs uniquely expressed in a given cell or tissue. e.g T cells and B cells will express many common RNAs, as well as a very small percentage which will be unique for T cells and B cells respectively. To make a T cell subtraction library, the cDNA from a T cell library is hybridized with a vast excess of B cell RNA. The commonly expressed genes will result in RNA-cDNA hybrids which can be removed (or subtracted) to leave only T cell specific cDNAs
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항상 말하듯이 본인은 답변의 정확성 및 내용에 대한 아무런 책임을 지지 않으며, 참고사항으로 답변을 제공합니다
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A cDNA library that only contains cDNAs uniquely expressed in a given cell or tissue. e.g T cells and B cells will express many common RNAs, as well as a very small percentage which will be unique for T cells and B cells respectively. To make a T cell subtraction library, the cDNA from a T cell library is hybridized with a vast excess of B cell RNA. The commonly expressed genes will result in RNA-cDNA hybrids which can be removed (or subtracted) to leave only T cell specific cDNAs
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항상 말하듯이 본인은 답변의 정확성 및 내용에 대한 아무런 책임을 지지 않으며, 참고사항으로 답변을 제공합니다
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